重组人干细胞因子对猕猴外周血干细胞的动员作用  

THE EFFECTS OF RECOMBINANT HUMAN STEM CELL FACTOR ON MOBILIZATION OF PERIPHERAL BLOOD STEM CELLS IN RHESUS MONKEYS

作　　者：熊国林[1] 郝静[1] 柳晓兰[1] 董波[1] 赵振虎[1] 邱丽玲[1] 陈松森[2] 雒蓬轶 王若竹 罗庆良[3] 

机构地区：[1]军事医学科学院放射医学研究所 [2]中国医学科学院基础医学研究所 [3]成都地奥九泓药厂

出　　处：《解放军医学杂志》2002年第8期706-708,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家"8 63"高技术基金资助课题 (编号 2 0 0 1AA2 1 540 1 );全军医学科研"九五"计划指令性课题 (编号 96L0 0 1 )

摘　　要：给正常成年猕猴每天一次皮下注射重组人粒细胞集落刺激因子 10 μg·kg-1·d-1(μKD)或 (和 )重组人干细胞因子 2 0、5 0和12 5 μKD ,前者给药 5天 ,后者 8天 ,观察国产重组人干细胞因子动员外周血干细胞的效果。结果表明 ,给药后外周血白细胞数最高值rhG CSF单独给药组为 2 0 6 % ,rhSCF单独给药为 2 0 0 % ,而两者联合给药后为 2 80 %～ 310 % ;外周血CFU GM较动员前分别升高 1 1、1 3和 5 8～7 9倍 ;3个联合动员组外周血CD34+ 细胞数分别为动员前的 2 0、2 4和 35倍。说明rhSCF对外周血干 /祖细胞有明显的动员作用 ,rhSCF+rhGTo evaluate the effects of recombinant human stem cell factor on mobilization of peripheral blood stem cells, 30 monkeys were divided into control, rhG CSF 10μg·kg -1 ·d -1 (μKD), rhSCF 50μKD, rhG CSF 10μKD+rhSCF 20μKD, rhG CSF 10μKD+rhSCF 50μKD and rhG CSF 10μKD+rhSCF 125μKD groups. The monkeys were given subcutaneous injections of rhSCF for 8 days and/or rhG CSF for 5 days. The highest value of leukocyte in rhG CSF and rhSCF groups was 206% and 200% respectively, and that of rhG CSF+rhSCF groups was 280% to 310%. CFU GM of peripheral blood was up to 1 1, 1 3 and 5 8～7 9 times respectively after the mobilization. CD34 positive cell number of joint mobilization group was 20, 24 and 35 times respectively of that before mobilization. rhSCF can mobilize the effect of peripheral blood stem cells, and the effect of joint rhSCF+rhG CSF is better.

关 键 词：重组人干细胞因子 外周血干细胞 动员作用 骨髓 造血干细胞 

分 类 号：R457.1[医药卫生—治疗学]