大麻药总皂苷中大麻药苷B的含量测定及其在体肠吸收特性分析  

Determination of Doliroside B in Total Saponins from Dolichos appendiculatus Roots and Analysis of Its in situ Intestinal Absorption Characteristics

作　　者：徐海燕[1] 覃勤[1] 葛月宾[1] 陈旅翼[1] 

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中国实验方剂学杂志》2015年第20期21-24,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81470181);湖北省自然科学基金群体项目(2013CFA013)

摘　　要：目的：建立大麻药苷B的HPLC分析方法,用于测定其在有效部位大麻药总皂苷中的含量,并考察大麻药苷B的大鼠在体肠吸收能力。方法：采用Ultimate AQ-C18色谱柱（4.6 mm×250 mm,5μm）,流动相0.1%乙酸乙腈溶液-0.1%乙酸水溶液梯度洗脱,流速0.5 m L·min-1,检测波长215 nm,进样量20μL。结果：标准曲线线性范围60~210 mg·L-1,相关系数0.999 9,加样回收率在95%~105%,4批大麻药总皂苷提取物中大麻药苷B质量分数分别为89.99%,82.08%,89.96%,84.38%。大鼠小肠在体肠吸收大麻药苷B的吸收速率常数（0.018 3±0.001 1）h-1,5 h累积吸收率（9.49±0.26）%.结论：该HPLC分析方法简便、准确,可用于大麻药苷B的含量测定。大麻药苷B在大鼠小肠内吸收情况较差。Objective： To develop a HPLC method for analyzing the content of doliroside B in total saponins from Dolichos appendiculatus roots,and to investigate intestinal absorption characteristics of doliroside B.Method： Ultimate AQ-C18column（ 4. 6 mm × 250 mm,5 μm） was used with a mobile phase consisted of wateracteonitrile containing 0. 1% acetic acid for gradient elution at a flow rate of 0. 5 m L·min-1,detection wavelength was 215 nm and an injection volume was 20 μL. Result： Good linearity was obtained over the concentration range of 60-210 mg·L-1with the correlation coefficient of 0. 999 9. The mean recoveries were between 95% and 105%.The content of doliroside B in four batches of total saponins extract from D. appendiculatus roots were all above80%. In addition,the absorption rate constant of doliroside B in small intestine was（ 0. 018 3 ± 0. 001 1） h-1and cumulative absorption percentage in 5 h was（ 9. 49 ± 0. 26） %. Conclusion： This HPLC method is simple and accurate for quality control of doliroside B. Doliroside B is badly absorbed in small intestine.

关 键 词：大麻药苷B 总皂苷 大麻药 肠吸收 大麻药苷A 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]