HPLC同时测定沉香中5种色酮含量  

Simultaneous Determination of Five Kinds of Chromone in Aguilariae Lignum Resinatum by HPLC

作　　者：杜海芳 熊礼燕[1] 林励[1] 帅欧[1] 祁龙凯 

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中国实验方剂学杂志》2015年第20期87-90,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家发改委高新技术产业化项目(国发办2011-51);惠州市科技计划项目(2010BO20010005)

摘　　要：目的：通过对沉香中5种色酮的含量测定,建立沉香质量控制方法。方法：采用HPLC法测定沉香中5种色酮含量,采用Waters C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈（A）-0.1%磷酸水（B）,流速1 m L·min-1,检测波长252nm,柱温30℃,进样量10μL。结果：色酮A,色酮B,色酮C,色酮D,色酮E{（5S,6R,7R,8S）-2-[2-（4-甲氧基-苯基）乙基]-5,6,7,8-四羟基-5,6,7,8-四氢色酮}分别在7.15~358.00,0.88~44.20,1.54~76.80,1.26~63.20,1.54~77.00μg与峰面积之间线性关系良好（r〉0.999 0）;精密度、重复性、稳定性的RSD均〈2%,平均加样回收率95.56%~98.82%。结论：该HPLC法可简单、快速、有效测定沉香中5种色酮的含量,可作为沉香质量控制方法。Objective： Through the content determination of the five kinds of chromone in Aguilariae Lignum Resinatum,to establish a method to control Aguilariae Lignum Resinatum quailty. Method： Determinate five kinds of chromone content by HPLC method,by the Waters C18column（ 4. 6 mm × 250 mm,5 μm）,mobile phase was acetonitrile-（ A）-0. 1% phosphorric acid（ B）,flow rate was 1 m L·min-1,the detection wavelength was 252 nm,the column temperature was 30 ℃,the sample volume was 10 μL. Result： The linear ranges of aquilarone B,aquilarone C,aquilarone D,aquilarone E {（ 5S,6R,7R,8S）-2-[2-（ 4-methoxy phenyl）ethyl]-5,6,7,8-four hydrox-y-5,6,7,8-four hydrogen chromone } were 7. 15-358. 00,0. 88-44. 20,1. 54-76. 80,1. 26-63. 20,1. 54-77. 00 μg respectively. The RSD of precision,reproducibility and stability tests were less than 2%,the average recoveries were ranged from 95. 56% to 98. 82 %. Conclusion： The determination of five kinds of chromone content in Aguilariae Lignum Resinatum by HPLC method is simple and fast,effective.Established a method to determinate five kinds of chromone content in Aguilariae Lignum Resinatum successful. It is provided a reference for Aguilariae Lignum Resinatum quality control.

关 键 词：沉香 色酮 含量测定 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]