西黄滴丸对S180荷瘤小鼠CD34及VEGF影响及形态学观察的实验研究  

Influence of Xihuang Dropping Pills on CD34 and VEGF of S180 Tumor-bearing Mice and Experience on Morphology

作　　者：车萍[1] 王中霞 李峰[1] 欧阳兵[3] 季旭明[1] 

机构地区：[1]山东中医药大学,济南250355 [2]山东宏济堂制药集团有限公司,济南250355 [3]山东省食品药品监督管理局,济南250011

出　　处：《中国实验方剂学杂志》2015年第20期95-98,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2010ZX09401)

摘　　要：目的:研究西黄滴丸对S180肉瘤小鼠的抑瘤作用,并探讨西黄滴丸对荷瘤小鼠肉瘤组织中血管生成标记物(CD34)和相关因子血管内皮生长因子(VEGF)表达的影响。方法:采用S180肉瘤小鼠模型,分为模型组,西黄丸组(2.2 g·kg-1·d-1),西黄滴丸低、中、高剂量组(1.5,3,6 g·kg-1·d-1)以及环磷酰胺组(CTX,0.02 g·kg-1),西黄丸组,西黄滴丸低、中、高剂量组ig给药,环磷酰胺组ip给药,每2 d 1次,连续10 d给药;观察西黄滴丸对S180荷瘤小鼠的抑瘤作用,并采用HE染色观察肿瘤细胞的形态变化,免疫组织化学的方法观察西黄滴丸对荷瘤小鼠肿瘤CD34及VEGF蛋白表达的影响。结果:与模型组比较,滴丸高剂量组荷瘤小鼠体重有明显升高;西黄丸组,西黄滴丸低、中、高剂量组及CTX组抑瘤率分别是26.28%,21.47%,26.92%,30.76%,35.85%;CD34的蛋白表达CTX组较模型组有明显降低(P<0.01),西黄滴丸高、中、低剂量组明显降低CD34蛋白表达(P<0.05),滴丸高剂量组较西黄丸组有较明显差异(P<0.05);滴丸中、高剂量组及CTX组与模型组比较VEGF的蛋白表达有明显降低(P<0.05),滴丸中剂量组及CTX组较西黄丸组蛋白表达有较明显差异。结论:西黄滴丸对S180荷瘤小鼠具有抑瘤的作用,其机制可能与下调肿瘤组织CD34及VEGF蛋白表达水平有关。Objective： To observe the antitumor effects of Xihuang dropping pills in S180 tumor-bearing mice and their influence on angiogenesis markers（ CD34） and vascular endothelial growth factor（ VEGF）expression in sarcoma tissues of tumor-bearing mice. Method： S180 tumor-bearing mice were divided into model group,Xihuang pills group（ 2. 2 g·kg-1·d-1）,Xihuang dropping pills low dose,middle dose and high dose groups（ 1. 5,3,6 g ·kg-1·d-1） and cytoxan group（ CTX,0. 02 g·kg-1）. Xihuang pills group,Xihuang dropping pills low dose,middle dose and high dose groups were administered ig,and CTX group was administered ip,1 time /2 days for continuous 10 days. The antitumor effects of Xihuang dropping pills in S180 tumor-bearing mice were observed,and the morphology changes of tumor cells were observed by HE staining. The influence of Xihuang dropping pills on CD34 and VEGF expression was observed by immunohistochemical method. Result：Compared with the model group,the body weight in tumor-bearing mice in Xihuang dropping pills high dose group was significantly increased. The anti-tumor rate in Xihuang pills group,Xihuang dropping pills low,middle,high groups and CTX group was 26. 28%,21. 47%,26. 92%,30. 76% and 35. 85% respectively. CD34 protein expression in CTX group and Xihuang dropping pills high dose group was significantly lower than that in model group（ P〈 0. 05,P 〈0. 01）. VEGF protein expression in Xihuang dropping pills middle,high dose group and CTX group was significantly lower than that in model group（ P 〈0. 05）. There was significant difference in proteins expression between Xihuang dropping pills middle dose group and Xihuang pills group. Conclusion：Xihuang dropping pills have the anti-tumor effect in S180 tumor-bearing mice,and the mechanism may be associated with down-regulating expression of CD34 and VEGF protein in tumor tissues.

关 键 词：西黄滴丸 西黄丸 S180 抑瘤 血管内皮生长因子 CD34 

分 类 号：R285.5[医药卫生—中药学]