益肾化浊方对Aβ_(25-35)介导神经干细胞增殖与分化的影响  

Effect of Yishen Huazhuo Decoction on Proliferation and Differentiation of Neural Stem Cells Mediated by Aβ_(25-35)

作　　者：韩文文[1,2] 张玉莲[1] 周震[1] 张琳琳[1] 王凯[2] 宋宛珊[2] 崔远武[1] 

机构地区：[1]天津中医药大学第二附属医院,天津300150 [2]天津中医药大学,天津300193

出　　处：《中国实验方剂学杂志》2015年第20期99-102,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81102577);国家重点基础研究发展计划(973计划)项目(2010CB530405)

摘　　要：目的:观察益肾化浊方对阿尔茨海默病(AD)脑中神经干细胞(NSCs)增殖分化的影响,为益肾化浊方治疗AD提供实验依据。方法:分离孕14 d小鼠胚胎NSCs,并以β淀粉样蛋白25-35(Aβ25-35)处理,分为空白组(正常培养基),模型组(5μmol·L-1Aβ25-35),Aβ25-35+益肾化浊方低剂量(0.1 mg·L-1)组,Aβ25-35+益肾化浊方中剂量(1 mg·L-1)组,Aβ25-35+益肾化浊方高剂量(10 mg·L-1)组,共5组,药物在Aβ25-35作用2 h后加入,培养至48 h后,进行检测。采用WST法检测益肾化浊方对NSCs增殖的影响,免疫荧光法检测其对NSCs分化的影响。结果:与空白组比较,模型组细胞活力明显降低,(GFAP+/DAPI)%比例上升,(Tubulin+/DAPI)%比例降低,差异具有统计学意义(P<0.05)。与模型组相比,益肾化浊方各剂量组均可以提高Aβ25-35损伤NSCs活力,降低(GFAP+/DAPI)%比例,升高(Tubulin+/DAPI)%比例,差异具有统计学意义(P<0.05),且均以低剂量组效果更为明显。结论:益肾化浊方可促进Aβ25-35介导的NSCs增殖,诱导其向神经元方向分化,抑制其向星形胶质细胞方向分化。Objective： To investigate the effects of Yishen Huazhuo decoction on the proliferation and differentiation of Alzheimer＇s disease（ AD） neural stem cells（ NSCs）,and to provide experimental basis for the treatment of AD by Yishen Huazhuo decoction. Method： NSCs were isolated from Kunming rats at embryonic day（ E） 14 and were processed with β-amyloid 25-35（ Aβ25-35）. The cells were randomly divided into blank group,model group（ 5 μmol·L-1Aβ25-35） and Aβ25-35＋ YSHZ decoction low dose group（ 0. 1 mg·L-1）,middle dose group（ 1 mg·L-1） and high dose group（ 10 mg·L-1）. The drug was added after 2 h of Aβ25-35 action,and the cells were detected after 48 h of culture. The effect of Yishen Huazhuo decoction on the proliferation of NSCs was observed by, WST method and its effect on differentiation was determined by immune fluorescence method.Result： Compared with the blank group,the cell activity in model group was significantly reduced,with increased（ GFAP＋/ DAPI） % and reduced（ Tubulin＋/ DAPI） %,with significantly statistical difference（ P〈 0. 05）,and the low dose group achieved the best effect. Conclusion： Yishen Huazhuo decoction promotes the proliferation of NSCs mediated by Aβ25-35,induces NSCs differentiation to neurons and inhibits their differentiation to astrocytes.

关 键 词：阿尔茨海默病 益肾化浊方 神经干细胞 神经元 星形胶质细胞 

分 类 号：R285.5[医药卫生—中药学]