芦荟大黄素联用顺铂对乳腺癌MDA-MB-231细胞增殖和凋亡的影响  

Effects of Aloe Emodin Alone and in Combination with Cisplatin on Cell Proliferation and Apoptosis of Breast Cancer Cell Line MDA-MB-231

作　　者：陶毅明[1] 洪雪 马义丽[1] 廖朝亮 

机构地区：[1]桂林医学院生物技术学院,广西桂林541004 [2]广东省肾脏病研究所,广州510515

出　　处：《中国实验方剂学杂志》2015年第20期127-130,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2012年度"广西高等学校优秀人才资助计划"项目(桂教人(2012)41号)

摘　　要：目的:探讨芦荟大黄素(AE)单用或联用顺铂(CP)对乳腺癌MDA-MB-231细胞增殖和凋亡的影响。方法:以MDA-MB-231细胞为研究对象,用5,10,20,30,40,50μmol·L-1AE,2,4,8,12,24,36μmol·L-1CP,或不同浓度AE联用CP处理乳腺癌MDA-MB-231细胞48 h,另设空白组,MTT法检测细胞增殖;Annexin V FITC/PI双染和流式细胞术检测细胞凋亡与细胞周期;Western blot分析Bcl-2相关X蛋白(Bax),B细胞淋巴瘤/白血病-2(Bcl-2)和Bcl-x L蛋白表达的变化。结果:与空白组比较,AE单用显著抑制乳腺癌细胞MDA-MB-231的增殖,半数抑制浓度(IC50)为22.21μmol·L-1,与CP联用时IC50为9.51μmol·L-1。联用指数CI50<1,表明两药物有协同作用;AE单用使细胞周期阻滞在G1期,并诱导细胞凋亡,联用CP则增强阻滞,凋亡率明显增加;两药联用显著抑制Bcl-2和Bcl-x L的表达,并上调Bax的表达,与空白组比较均具有明显的统计学差异(P<0.05,P<0.01)。结论:芦荟大黄素抑制乳腺癌MDA-MB-231细胞增殖,使细胞周期阻滞在G1期,并通过上调促凋亡蛋白和下调抗凋亡蛋白,诱导凋亡。与顺铂联用能增强其促凋亡作用,两药存在协同作用。Objective： To investigate the anti-proliferation and apoptosis effects of aloe emodin（ AE）alone and in combination with cisplatin（ AE-CP） on breast cancer cell MDA-MB-231. Method： MDA-MB-231 cells were treated for 48 h with 5,10,20,30,40,50 μmol·L-1AE,2,4,8,12,24,36 μmol·L-1CP or various concentrations of AE-CP. Cell proliferation was detected by MTT. Apoptosis and cell cycle were detected by Annexin V FITC / PI double-staining and flow cytometer. The protein levels of Bax,Bcl-2 and Bcl-x L were analyzed by Western blot. Result： AE alone significantly inhibited cell proliferation,with an IC50 of 22. 21 μmol·L-1. IC50 decreased to 9. 51 μmol·L-1when it was used in combination with CP. The combination index CI50 1,indicating a synergic effect. AE alone arrested cell cycle in G1 phase and induced apoptosis. The arrest was accentuated when CP was given simultaneously,accompanied by a marked increment of apoptosis rate. AE-CP significantly inhibited the protein levels of Bcl-2 and Bcl-x L,but increased Bax. Conclusion： AE could inhibit the cell proliferation of MDA-MB-231,arrest the cell cycle in G1 phase,and induce apoptosis by increasing apoptosis proteins and decreasing anti-apoptosis proteins. AE shows a synergic effect of apoptosis when combined with CP.

关 键 词：芦荟大黄素 乳腺癌 MDA-MB-231细胞 凋亡 顺铂 

分 类 号：R285.5[医药卫生—中药学]