乌蛇驱风汤及诸加减方对豚鼠慢性湿疹模型的疗效比较  

Efficacy Comparison of Wushe Qufeng Decoction and Its Derivative Prescriptions in Chronic Eczema Model of Guinea Pigs

作　　者：林靓[1] 滕浦陵 许日红 

机构地区：[1]安徽中医药高等专科学校,安徽芜湖241000 [2]芜湖市中医院,安徽芜湖241000

出　　处：《中国实验方剂学杂志》2015年第20期137-140,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：省级优秀青年人才基金项目(2009SQRZ215)

摘　　要：目的:比较乌蛇驱风汤及诸加减方对豚鼠慢性湿疹的疗效。方法:健康豚鼠58只,随机抽取8只,雌雄各半,作为正常组,将其余的50只豚鼠参照进行造模,背部剃毛,小剂量2,4-二硝基氯苯为抗原,多次刺激豚鼠皮肤制备慢性湿疹模型,将造模成功的48只豚鼠分雌雄随机分为6组,分别为1号方组(乌蛇驱风汤原方,8.05 g·kg-1),2号方组(7.22 g·kg-1),3号方组11.75 g·kg-1,4号方组(12.86 g·kg-1),丁酸氢化可的松乳膏组(0.5 g·kg-1)、模型组,口服ig给药,连续给药7 d,末次用药后24 h,处死所有实验动物,根据皮损局部的症状评分和皮损处的病理改变进行疗效评估。结果:与正常组比较,模型组病理症状评分明显升高(P<0.05),模型组皮损处的病理表现仍呈现表皮和真皮水肿,表皮层增厚,以棘层细胞增生为主,真皮浅层血管轻度扩张,周围有以淋巴细胞为主的炎症细胞浸润;与模型组比较,1号方组,2号方组,3号方组,4号方组及丁酸氢化可的松乳膏组病理症状评分明显降低(P<0.05),乌蛇驱风汤组及丁酸氢化可的松乳膏组皮损组织病理改变较模型组明显改善,与氢化可的松组病理表现相似;各乌蛇驱风汤加减方组组织病理表现较模型组改善不明显。结论:乌蛇驱风汤对豚鼠慢性湿疹的疗效优于诸加减方,与丁酸氢化可的松乳膏组疗效相当。Objective： To compare the efficacy of Wushe Qufeng decoction and its derivative prescriptions in chronic eczema of guinea pigs. Method： Eight guinea pigs（ 4 female and 4 male） were randomly selected from 58 healthy guinea pigs as the normal group. The remaining 50 guinea pigs were used to make the chronic eczema models by irritating the skins of their shaved backs with low-dose 2,4-dinitrochlorobenzene as the antigen for repeated stimulation. 48 successfully modeled ones were randomly divide into 6 groups： No. 1prescription group（ Wushe Qufeng decoction,8. 05 g·kg-1）,No. 2 prescription group（ 7. 22 g·kg-1）,No.3 prescription group（ 11. 75 g · kg-1）,No. 4 prescription group（ 12. 86 g·kg-1）,hydrocortisone butyrate cream group（ 0. 5 g·kg-1）,and model group. The guinea pigs in these groups were given orally for 7 days. 24 h after the last administering,all experiment animals were sacrificed,and the efficacy was assessed according to the symptom scores in local skin lesions and pathological changes of the skin lesions. Result： Symptom score：compared with normal group,the pathological symptom scores in model group were significantly higher（ P〈 0. 05）. Compared with model group,the pathological symptom scores in No. 1 prescription group,No. 2prescription group,No. 3 prescription group,No. 4 prescription group and hydrocortisone butyrate cream group were significantly reduced（ P〈 0. 05）. The pathological changes in skin lesions were significantly improved in Wushe Qufeng decoction group and hydrocortisone butyrate cream group,similar to those in drug control group. No obvious difference was found between the groups of the derivative prescriptions of the Wushe Qufeng decoction and the model group. Conclusion： The efficacy of the Wushe Qufeng decoction in the chronic eczema of guinea pigs is better than that of its derivative prescriptions,equaling to that of the drug control group.

关 键 词：乌蛇驱风汤 慢性湿疹 豚鼠 

分 类 号：R285.5[医药卫生—中药学]