二冬膏对急性肺损伤模型鼠TNF-α,IL-6含量及AQP-5蛋白表达的影响  

Effect of Erdong Gao on TNF-α,IL-6 and AQP-5 in Rats with Acute Lung Injury

作　　者：熊秋迎[1] 陈乔[2] 谢斌[2] 朱金华[2] 孙昊鑫[2] 何莲花[2] 

机构地区：[1]南昌大学第一附属医院,南昌330006 [2]江西中医药大学,南昌330004

出　　处：《中国实验方剂学杂志》2015年第20期167-170,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西中医药大学科研基金资助(2013ZR0052)

摘　　要：目的:研究二冬膏对脂多糖(LPS)致急性肺损伤(ALI)模型鼠肿瘤坏死因子-α(TNF-α),白介素-6(IL-6)含量及水通道蛋白5(AQP-5)表达的影响。方法:将72只清洁级SD大鼠随机分为6组:正常组、模型组、二冬膏高、中、低剂量组、地塞米松组,每组12只。除正常组外,其余各组采用一次性尾静脉注射LPS 6 mg·kg-1复制ALI大鼠模型。首次给药在造模后0.5 h,1次/d,连续4 d。二冬膏高、中、低剂量组分别ig二冬膏10,5,2.5 g·kg-1;正常组与模型组ig生理盐水10 m L·kg-1,地塞米松组ip地塞米松0.01 g·kg-1。4 d后处死大鼠,取血清和肺组织检测。采用双抗体夹心-酶联免疫吸附(ABC-ELISA)法检测大鼠血清TNF-α,IL-6的含量,Western bloting测定大鼠肺组织AQP-5蛋白的表达,苏木素和伊红(HE)染色观察肺组织病理形态。结果:与正常组比较,模型组大鼠血清TNF-α,IL-6含量明显升高(P<0.01),肺组织AQP-5蛋白表达水平显著降低(P<0.01)且肺部病理炎症增加。与模型组比较,二冬膏各组均能明显降低TNF-α,IL-6含量(P<0.05,P<0.01),显著升高肺组织AQP-5蛋白的表达(P<0.05,P<0.01)并且减轻肺部病理炎症。结论:二冬膏通过降低ALI模型鼠促炎因子TNF-α,IL-6含量,升高肺组织AQP-5蛋白的表达,改善肺部病理炎症,减轻肺水肿状态,从而有效防治急性肺损伤。Objective： To study the effect of Erdong Gao on tumour necrosis factor-α（ TNF-α）,interleukin-6（ IL-6） and aquaporin-5（ AQP-5） in rats with acute lung injury（ ALI） induced by lipopolysaccharide（ LPS）. Method： Seventy-two SD rats were randomly divided six groups： normal group（ A）,model group（ B）,Erdong Gao high-dose group（ C）,Erdong Gao medium-dose group（ D）,Erdong Gao low-dose group（ E） and dexamethasone group（ F）,twelve rats in each group. All the other groups except normal group received disposably iv LPS 6 mg ·kg-1through tail vein to establish ALI rat model. The rat＇s received first medication 0. 5 hour after modeling,once a day,lasting for 4 days. The rats of groups C,D,E were respectively ig given with Erdong Gao（ 10,5,2. 5 g · kg-1）; the rats of groups A and B were given with ip 10 m L·kg-1normal saline; the rats of group F were given with ip 0. 01 g·kg-1dexamethasone. After 4 days,the rats were sacrificed,and serum and lung tissue were taken for testing. The contents of TNF-α,IL-6 in serum were detected by double antibody clip enzym-linked immunosorban assay（ ABC-ELISA） method. The expression of AQP-5 in lung tissue was determined by Western bloting method. The pathological morphology of lung tissue was observed by hematoxylin and eosin（ HE） staining. Result： Compared with group A,the contents of TNF-α,IL-6 in serum were obviously increased（ P〈0. 01）; expression of AQP-5 in lung tissue was significantly decreased（ P〈0. 01）and the pathological inflammation in lung tissue became serious in rats of group B. Compared with group B,Erdong Gao could significantly reduce the content of TNF-α,IL-6 in serum（ P〈0. 05,P〈0. 01）,increase the expression of AQP-5 in lung tissue（ P〈0. 05,P〈0. 01）,and alleviate the pathological inflammation in lung tissue.Conclusion： Erdong Gao could reduce the content of proinflammatory factor TNF-α,IL-6 and increase the expression of AQP-5 in lung tissue,improve the pathological infla

关 键 词：二冬膏 脂多糖 急性肺损伤 肿瘤坏死因子-α 白介素-6 水通道蛋白-5 

分 类 号：R285.5[医药卫生—中药学]