随机扩增多态性DNA在细菌鉴定分型中的影响因素  

THE INFLUENTIAL FACTORS AND COMPARABILITY AMONG LABORATORIES OF RAPD ON THE IDENTIFICATION AND GENOTYPING OF BACTERIA

作　　者：赵蕊[1] 王军[1] 李跃旗[1] 石建时[1] 

机构地区：[1]解放军第302医院

出　　处：《解放军医学杂志》2002年第8期724-725,共2页Medical Journal of Chinese People's Liberation Army

摘　　要：为探讨各种试剂的浓度及不同DNA扩增仪对随机扩增多态性DNA(randomlyamplifiedpolymorphicDNA ;RAPD)的影响因素。用不同浓度引物、4×dNTP等试剂及 3种不同型号DNA扩增仪对 11株致病弧菌进行RAPD扩增。结果 11株致病弧菌分成 4型 ,重复性好。不同引物、4×dNTP对RAPD构成一定影响 ,3种DNA扩增仪产生的DNA指纹图谱基本一致。提示用于RAPD的试剂可以标准化 。To investigate the influential factors of different concentrations of some reagents and the comparability among the laboratories of RAPD. RAPD was carried out with different concentrations of reagents and three different amplified cyclers to amplify 11 strains of pathogenic vibrios. 11 strains of pathogenic vibvios were genotyped into 4 types, and could be easily repeated.The different concentrations of primer and 4 dNTP could influence the result of RAPD,but the fingerprints of RAPD produced respectively with 3 amplified cyclers were consistent. It is suggested that RAPD can be compared among the laboratories with the standard reagents.

关 键 词：影响因素 随机扩增多态性DNA技术 细菌分技术 质量控制 

分 类 号：R378[医药卫生—病原生物学] Q503[医药卫生—基础医学]