PRRSV河北分离株ORF5与NSP2基因遗传变异分析  

Genetic variation analysis of ORF5 and NSP2 genes of porcine productive and respiratory syndrome virus isolate from Hebei province

作　　者：孙明潭 左奕[1,2] 袁万哲[1,2] 孙继国[1,2] 

机构地区：[1]河北农业大学动物医学院,河北保定071001 [2]河北省兽医生物技术工程技术研究中心,河北保定071001

出　　处：《中国兽医学报》2015年第10期1578-1583,共6页Chinese Journal of Veterinary Science

基　　金：河北省畜牧局科技资助项目(2014-3-03)

摘　　要：从河北省新乐市某猪场采集疑似感染猪繁殖与呼吸综合征病毒(PRRSV)的病料,通过Marc-145细胞培养,RT-PCR检测等方法鉴定,确认分离到1株PRRSV,暂命名为HB-XL株。进一步对其ORF5与NSP2基因克隆测序,经同源性比较及系统进化树等分析,表明该毒株ORF5与NSP2基因与国内流行的缺失变异株遗传关系较近,与国内外经典美洲型毒株及基因重组毒株QYYZ遗传关系较远且与欧洲型LV毒株处于不同分支;ORF5基因编码的200个氨基酸的第13、151位均为具有强毒特性的精氨酸(R),第137位为具有野毒特性的丝氨酸(S);与经典美洲株相比,该毒株NSP2基因分别发生3个碱基和87个碱基的不连续缺失。说明HB-XL株属于美洲型PRRSV并存在不断变异的趋势。A porcine productive and respiratory syndrome virus（PRRSV）field strain,tentatively named HB-XL,was isolated from suspicious PRRSV-positive materials of sick pigs in Xinle areas of Hebei province,according to the Marc-145 cell culture and RT-PCR detection method.Further analysis by cloning ORF5 and NSP2sequencing,homology comparison and phylogenetic evolutionary tree showed that the ORF5 and NSP2of HB-XL share the similar mutant genetic relationship to the majority of the domestic epidemic PRRSV strains,and the relatively distant relationship to the American classical strains and recombinant strain QYYZ,and the Europe strain LV is an independent branch;The ORF5 gene consisted of 200 amino acids and the sites of 13 and 151were argrinine（R）which were related to virulent strains,and the site of 137 was serine（S）indicating that the isolated strain was wild strain;the Nsp2 gene had a discontinuous deletions of 3bases and 87 bases compared with the American classical strains.The results showed that PRRSV HB-XL strain belongs to the American type PRRSV,and with a trend of continuous variation.

关 键 词：PRRSV 分离鉴定 NSP2 ORF5 变异分析 

分 类 号：S852.651[农业科学—基础兽医学]