藏药绿萝花抗氧化作用实验研究  

Research on tissue antioxidant effect of Tibetan medicine Scindapsus aureus

作　　者：孙翠翠[1] 康莲莲[1] 王赛[1] 韩金潭 杨荣[1] 刘群[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《中兽医医药杂志》2015年第5期18-23,共6页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家"十二五"科技支撑计划项目(2011BAD34B02-4);西南民族大学研究生创新型科研项目

摘　　要：研究藏药绿萝花抗氧化作用,为临床合理用药和开发利用提供科学依据。采用注射D-gal法模拟小鼠衰老进程,并将实验动物分成空白组、模型组、绿萝花高剂量组(2 g/m L)、绿萝花中剂量组(1 g/m L)、绿萝花低剂量组(0.5 g/m L),分别在实验的第0、7、14、21、28、35、42天,以小鼠血清SOD、MDA为动态指标及42 d大脑、心、肝组织SOD、MDA、T-AOC、GSH-Px、MAO、CAT为检测指标,研究绿萝花的抗氧化作用。结果表明,连续注射D-gal 14-42 d,模型小鼠血清SOD活性下降、MDA含量上升;连续同时服用绿萝花口服液14 d后小鼠血清SOD活力显著提高,以低剂量绿萝花作用最显著;连续服用绿萝花14 d后小鼠血清MDA含量显著降低,以高剂量绿萝作用最显著;连续服用绿萝花42 d后,小鼠血清T-AOC活力升高,大脑、心、肝SOD、T-AOC、CAT、GSH-Px活性上升,MAO活性下降,MDA含量下降。结果表明,连续口服绿萝花能改善由注射D-gal引起的小鼠抗氧化指标的异常,提示绿萝花可能具有抗机体衰老作用。Objective： To study the antioxidant effect of Scindapsus aureus and provide scientific basis for rational drug use and utilization. Methods： Senile mice model was established by using D-gal injection. The mice were divided into blank group（treated with NS）, D-gal group（treated with D-gal）, Scindapsus aureus extract high, medium and low dose group（treated with D-gal combined with Scindapsus aureus extract at 2 g/m L, 1 g/m L and 0.5 g/m L,respectively）. The experiment lasted 6 weeks. The indicators in mice serum and tissues such as superoxide dismutase enzyme（SOD）, malondialdehyde（MDA）, total antioxidant capacity（T-AOC）, CAT and GSH-Px were detected on 0^th, 7^th, 14^ th, 21^ st, 28 ^th, 35^th and 42^nd, respectively. Results： Continuous injection of D-gal for 14-42 d, the activity of serum SOD was decreased and MDA content was increased; continuous administration of Scindapsus aureus extract could significantly increase serum SOD activity, especially in low-dose-treated group on 14^th; continuous administration of Scindapsus aureus extract could significantly decrease the content of MDA in serum,especially in high-dose-treated group on 14^th; continuous administration of Scindapsus aureus extract could significantly increase serum T-AOC activity, SOD, T-AOC, CAT and GSH-Px activity in brain, heart and liver, while decrease MAO activity and MDA content. Conclusion： Continuous administration of Scindapsus aureus extract can improve the aging indices of mice abnormal conditions caused by injection of D-gal, suggesting that Scindapsus aureus may be used as antioxidant.

关 键 词：藏药绿萝花 小鼠衰老模型 抗氧化作用 

分 类 号：S853.7[农业科学—临床兽医学]