响应面法优化闪式提取滁菊总黄酮的工艺  

Response surface methodology for the optimization of flash-extraction process of total flavonoids from Chuzhou Chrysanthemum

作　　者：刘峰[1] 武义来 时维静[1] 孙洁[1] 李响[1] 王茜[1] 缪成贵[1] 

机构地区：[1]安徽科技学院食品药品学院,安徽凤阳233100

出　　处：《中兽医医药杂志》2015年第5期23-28,共6页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家自然科学基金项目(81302783);国家级大学生创新创业训练计划项目(AH201310879023;201410879006);安徽科技学院第十二批大学生创新课题(15XSZ93;15XSZ95);科技部星火计划(2011GA710073);安徽省中药材产业技术研发中心基金资助(ZYC20101503);滁州市科技局"高校发明专利技术研究项目"(201305)

摘　　要：利用响应面法优化闪式提取滁菊总黄酮的最佳提取工艺。比较温浸法、超声波提取法和闪式提取法对滁菊总黄酮提取率的影响;在单因素试验基础上,采用响应面法对闪式提取溶剂浓度、料液比、提取时间进行优化,从而得到最佳的提取工艺条件。结果表明,闪式提取滁菊总黄酮最佳提取工艺为提取时间103 s,乙醇浓度62%,料液比1∶44,此条件下滁菊总黄酮提取率为6.90%。To optimize flash-extraction process of total flavonoids from Chuzhou Chrysanthemum by response surface methodology. Soaking extraction, ultrasonic extraction and flash extraction were compared. Based on single factor experiments,extraction conditions, such as solvent concentration, liquid/material ratio and extraction time were optimized by response surface methodology. The results showed that the three extraction conditions could significantly affect flavonoid extraction. The optimized conditions were as follows： 62% of ethanol concentration, 44 ∶1 of liquid/material ratio, and extracting for 103 s,resulting in an extraction efficiency of 6.90% for flavonoids. As a conclusion, flash-extraction is an effective and efficient method to extract flavonoids from Chrysanthemum.

关 键 词：响应面法 闪式提取 滁菊 总黄酮 

分 类 号：S853.7[农业科学—临床兽医学]