贵州烟草马铃薯Y病毒株系分析  

Analysis on strains of tobacco potato virus Y in Guizhou province

作　　者：王莉爽 陈文 谭清群 吴石平 袁洁 

机构地区：[1]贵州省植物保护研究所,贵阳550006

出　　处：《南方农业学报》2015年第8期1415-1419,共5页Journal of Southern Agriculture

基　　金：中国烟草总公司贵州省公司科技项目(201022)

摘　　要：【目的】探明贵州省烟草主产区马铃薯Y病毒（PVY）的主要株系,为制定烟草PVY的综合防治策略提供理论依据。【方法】采用双抗体夹心酶联免疫吸附法（DAS-ELISA）对从贵州省24个县（市）48个乡（镇）采集到的254份烟草病毒病疑似样品进行检测,每个乡镇选出一个代表性PVY阳性样品运用生物学方法并依据pl基因和cp基因序列联合分析鉴定PVY株系。【结果】源于贵州省24个县（市）的48个烟草PVY分离物与已知PVY分离物的p1基因和cp基因的核苷酸相似性在84.1%-98.6%,氨基酸序列同源性为86.1%-99.1%。其中与PVYN株系核苷酸相似性最高,达98.6%,氨基酸同源性为99.1%;其次是PVY^N∶O株系,核苷酸相似性为97.0%,氨基酸同源性为97.8%。由构建的系统发育进化树可知,17个县（市）的34条PVY分离物属于PVY^N∶O株系,7个县（市）的14条PVY分离物属于PVYN株系。【结论】贵州省烟草主产区PVY已具有明显的株系分化现象,且有一定的地域性。【Objective】The primary strains of potato virus Y（PVY） in the main producing areas of tobacco in Guizhou province were investigated, in order to provide theoretical basis for generating integrated control strategies for PVY.【Method】The 254 suspected tobacco samples infected by PVY were detected by double antibody sandwich enzyme-linked immunosorbent assay（DAS-ELISA）, which were collected from Guizhou province. A representative PVY positive sample was chosen from each of 48 towns, respectively. Then, PVY strains were identified by using biological method based on cp and p1 genes. 【Result】The reshults showed that the p1 and cp genes of 48 PVY isolates derived from 24 cities（towns） in 7 districts of Guizhou shared 84.1%-98.6% similarity of nucleotide sequence and 86.1%-99.1% homology of amino acid sequence with known PVY isolates. Furthermore, among 48 PVY isolates, the nucleotide similarity with PVYN strain were the highest（98.6%）, and the amino acid sequence homology with PVYNstrain were the highest（99.1%）,followed by 97.0% similarity of nucleotide with PVY^N∶Ostrain and 97.8% homology of amino acid sequence. In addition,the established phylogenetic tree showed that 34 PVY isolates from 17 cities（towns） belonged to PVY^N∶Ostrain, and 14 PVY isolates from other 7 cities（towns） belonged to PVYNstrain. 【Conclusion】The PVY in tobacco main producing areas of Guizhou shows obvious regional strain differentiation.

关 键 词：烟草 马铃薯Y病毒 病毒株系 分子变异 

分 类 号：S432.1[农业科学—植物病理学]