芝麻素对大鼠心肌缺血再灌损伤的保护作用  

Protective effect of sesamin on myocardial ischemia reperfusion injury in rats

作　　者：涂鹏程[1] 郑书国[2] 杨解人[2] 任尤楠 陶善珺 赵梦秋[2] 曹雨朦 许梦情 李方圆[1] 

机构地区：[1]皖南医学院临床医学院,安徽芜湖241002 [2]皖南医学院药理学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2015年第5期416-418,共3页Journal of Wannan Medical College

基　　金：国家级大学生创新创业训练计划项目(201410368046)

摘　　要：目的:探讨芝麻素对缺血再灌所致大鼠心肌损伤的保护作用及其可能机制。方法:SD大鼠50只随机分为正常对照组、模型组、假手术组、芝麻素低剂量组[80 mg/(kg·d)]及芝麻素高剂量组[160 mg/(kg·d)],每组10只。芝麻素组灌胃给予芝麻素7 d,每日1次,结扎冠状动脉左前降支建立心肌缺血再灌模型;假手术组大鼠只穿线,不接扎。HE染色观察心肌病理学变化,比色法测定血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平及心肌组织MDA、SOD水平。结果:芝麻素明显改善缺血再灌所致大鼠心肌损伤,减轻细胞水肿、变性、坏死及心肌间质炎症细胞浸润,降低血清CK、LDH水平(P<0.05或P<0.01)。芝麻素显著提高大鼠血清和心肌组织SOD活力,降低MDA水平(P<0.05或P<0.01),改善缺血再灌诱导的氧化应激。结论:芝麻素可明显改善缺血再灌所致大鼠心肌损伤,其机制与提高机体抗氧化能力、减轻缺血再灌所致氧化应激损伤有关。Objectlve：To investigate the protective effect of sesamin and its potential mechanisms against myocardial ischemia reperfusion injury in rats. Methods ： Fifty SD rats were randomly divided into 5 groups ： namely control group, model group, sham group, low dose of sesamin group [ 80mg/（ kg · d） ] and high dose of sesamin group [ 80 mg,/（ kg · d） ]. Rats in sesamin groups were intragastrically administered with sesamin for 7 d（qd）. Then all rats ex- cept those in control and sham groups were subjected to myocardial ischemia for 40 min by coronary artery ligation and subsequent reperfusion for 120 min. Rats in the sham group underwent the same operation without occlusion. Pathological changes of myocardial tissue were examined by hemotoxylin and eosin （HE） staining. Calorimetric assay was performed to determine the serum levels of creatine kinase （CK） and lactate dehydrogenase （LDH）, oxidative stress indexes including superoxide dismutase （SOD） activity and malondyaldehyde （MDA） content in serum and myocardial tissues. Results ： Pretreat- ment with sesamin evidently ameliorated myocardial injury induced by ischemia and reperfusion in rats as confirmed by histopathological examination and reduced serum levels of LDH and CK activity （ P 〈 0.05 or P 〈 0. 01 ）. Supplementation with sesamin resulted in a significant increase of SOD activity and decrease of MDA content in serum and myocardial tissues （ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion ： Sesamin effectively attenuated myocardial ischemia reper- fusion injury in rats,of which the underlying mechanisms might involve enhancement of antioxidant capacity.

关 键 词：心肌缺血再灌损伤 芝麻素 氧化应激 抗氧化剂 

分 类 号：R285.5[医药卫生—中药学]