系统性红斑狼疮患者Th1/Th2细胞及白介素12、18及其受体mRNA的表达  

Expression of Th1/Th2and Their Regu latory Cytokines in Systemic Lupus Erythematosus

作　　者：黎莉[1] 王晓栋[1] 陈顺乐[1] 沈南[1] 鲍春德[1] 顾越英[1] 

机构地区：[1]上海第二医科大学附属仁济医院上海市风湿病研究所,200001

出　　处：《中华皮肤科杂志》2002年第4期263-265,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(39970696)

摘　　要：目的探讨未经药物治疗的初发系统性红斑狼疮患者Th1/Th2细胞亚群分布,白介素12、白介素18及其受体的基因表达。方法运用三色荧光标记流式细胞术检测35例初发SLE患者、10例正常人Th1/Th2细胞亚群分布;ABI7700Real-TimePCR法同时检测38例患者和28例正常人IL-12、IL-18及其受体mRNA表达水平。结果①初发狼疮患者Th1较正常人明显减低(P<0.05),但Th1/Th2无显著性改变。②与正常人组相比,SLE组患者IL-12、IL-18mRNA及其受体表达较正常人明显降低(P值均<0.05);③面部红斑组患者Th1/Th2、IL-12P35较正常人组降低(P均<0.05);④RNP阳性组患者IL-12P40较正常人组升高,IL-12P35、IL-18较正常人组降低(P均<0.05)。结论SLE是一种以Th1细胞下降,Th2细胞相对占优势的自身免疫性疾病,源于诱导向Th1细胞分化的一系列细胞因子及其受体减少和细胞因子间失衡所致。Objective To analyze Th1/Th2subsets and their regulatory cytokines and cytokine r eceptors in recent onset SLE patients to determi ne the cause of immunological imbala nce in SLE.Methods Th1/Th2cells were detected quantitatively by flo w cytometry in patients with recent-onset SLE(n=35)and the controls(n=10).At the same time,IL -12,IL -12Rβ 2 and IL -18,IL -18R mRNA expression we re tested by ABI7700real time PCR in SLE patients(n=38/42)and the controls(n=28).Results No difference was found in Th1/Th2ra tio between SLE patients and the controls(P>0.05).However,Th1was decreased significantly in S LE patients than that in the normal controls(P<0.05).The cytokines including IL -12P35,IL -12P40?IL -18and their respective receptors IL -12Rβ 2 ?IL -18R and IL -12P35/IL -10?IL -18/IL -10were all significantly decreased in SLE group than those in the controls(P <0.05all).SLEpatients with facial erythema presented significantly lower Th1/Th2ratio and lower IL -12P35mRNA exp ression than the controls did.Signi ficantly high IL -12P40expression a s well as low IL -12P35expression were found in SLE patients with positive RNP.Conclusions SLE is a disease with Th2predominance due to low Th1s ubset and decreased expressions of I L -12and IL -12Rβ 2 ,IL -18and IL -18R mRNA.SLE patients with facial erythema and positive RNP may be the special subtype of SLE,for they have different Th distribution and the particular imbalance in cytokines exp ression.[

关 键 词：系统性红斑狼疮 TH1细胞 TH2细胞 白细胞介素12 白细胞介素18 白细胞介素受体 SLE 

分 类 号：R593.241[医药卫生—内科学]