肿瘤坏死因子α对角质形成细胞产生白介素8和增殖活性的影响  

Effects of TNF-α,sTNFRⅠon IL-8Production and Proliferation of Psoriatic Keratinocytes

作　　者：陈先进[1] 张开明[1] 尹国华[1] 李慧仙[1] 

机构地区：[1]山西省太原市中心医院皮肤科,现在山东省烟台毓璜顶医院264000

出　　处：《中华皮肤科杂志》2002年第4期293-295,共3页Chinese Journal of Dermatology

摘　　要：目的了解肿瘤坏死因子α(TNF-α)、肿瘤坏死因子受体Ⅰ(TNFRⅠ)和抗TNFRⅠ抗体对银屑病患者角质形成细胞(KC)产生白介素8(IL-8)和增殖活性的影响。方法分离表皮细胞接种96孔板,分别为:①自然增殖孔;②TNF-α刺激孔;③sTNFRⅠ/TNFα培养孔;④sTNFRⅠ/抗TNFRⅠ/TNF-α培养孔;⑤抗TNFRⅠ/TNF-α培养孔。ELISA法测上清液IL-8的含量;MTT法测增殖活性;收集细胞提取RNA进行反转录和扩增,产物电泳后转膜,进行DNA印迹以比较IL-8的表达在转录水平上的差异。结果银屑病患者组所测3个指标均较正常对照组高(P<0.05);两组TNF-α刺激孔KC3个指标均显著高于自然增殖孔和sTNFRⅠ/抗TNFRⅠ/TNF-α培养孔;自然增殖孔和sTNFRⅠ/抗TNFRⅠ/TNF-α培养孔之间比较差异无显著性。结论①银屑病患者KC处于活化状态,分泌高水平细胞因子且增殖活跃;②TNF-α可诱导体外培养KC表达IL-8mRNA和蛋白,促进KC增殖,其作用可能主要是通过和细胞表面TNFRⅠ结合来实现;③sTNFRⅠ可以部分阻断TNF-α的生物学作用。Objective To study the effects of TNF -αand sTNFRⅠon IL -8production and proliferatio n of psoriatic keratinocytes.Methods In vitro cultured keratinocytes were treated with TNF -α,sTNFRⅠand an-ti -TNFRⅠ.IL -8levels in supernatants were me asured by enzyme-linked immunosobent assay.Expression of IL -8mRNA in keratinocytes was observed by semiquantitive RT -PCR and Southern blotting.Proliferation index wa s measured by MTT colorimetry.Results IL -8levels,mRNA expression and pro liferation index were significantl y higher in psoriatic patients than th ose in control group(P<0.05).IL -8production and proliferation index of keratinocytes were significantly h igher in TNF -αtreated group than those in control group(P<0.05).No sig-nificant difference was found in IL -8production and keratinocyte proliferation between sTNFRⅠ/anti -TNFRⅠ/TNF -αtreated group and control group.Conclusions Activated keratinocytes secrete ab undant cytokines crucial to the development of psoriatic lesions.By binding to TNFRⅠrather than TNFRⅡon the membrane surface,TNF -αenhances IL -8production and proliferation of keratinocytes,and sTNFRⅠpartly neutralizes functions of TNF-α.[

关 键 词：银屑病 肿瘤坏死因子Α 白细胞介素8 角蛋白细胞 发病机制 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]