用非洲爪蟾分析新调控子PLUNC-p组织调控特性  

The regulatory activity of the lately cloned promoter PLUNC-p in transgenic Xenopus Laevis

作　　者：张玲[1] 冯湘玲[1] 周文[1] 李虹[1] 史建凌[1] 姚开泰[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所卫生部癌变重点实验室,湖南长沙410078

出　　处：《中国病理生理杂志》2002年第8期881-883,I001,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金重点资助项目 (No.39730 2 0 0 );国家自然科学基金资助项目 (No .39970 367)

摘　　要：目的 :构建含新克隆的启动子 (PLUNC -p)与增强型绿色荧光蛋白 (EGFP)的表达载体 ,研究该启动子的组织调控特性。方法 :爪蟾转基因系统。结果 :荧光显微镜下观察 ,与广泛性调控子巨细胞病毒 (cy tomegalovirus,CMV)启动子相比 ,PLUNC -p较为特异性地调控绿色荧光蛋白表达于爪蟾幼体的筛弓区及部分表皮。结论 :PLUNC -p很有可能具有上皮组织特异性调控特性 ,这一特性为实现转基因定向表达于上皮组织提供了可能。AIM: Our study focused on investigation of tissue specific regulatory activity of the newly cloned promoter: PLUNC-p with driving enhanced green fluorescent protein ( EGFP ). METHODS: Transgenic Xenopus Laevis system was applied.RESULTS: The green fluorescence protein directed under PLUNC-p was expressed strictly in branchial arches and epidermis of Xenopus Laevis embryos while CMV promoter showed ubiquitous regulation characteristic.CONCLUSION: PLUNC-p is able to direct epithelia specific expression of EGFP . This property of PLUNC-p might raise the possibility that lead target genes to express tissue-specifically.

关 键 词：PLUNC-P 转基因爪蟾 基因 启动区 启动子 增强型绿色荧光蛋白 基因调控 

分 类 号：Q78[生物学—分子生物学]