钙调神经磷酸酶在大鼠不同组织中的分布及活性  

Distribution and activity of calcineurin in different tissues of rat

作　　者：符民桂[1] 陈亚红[2] 刘秀华[3] 庞永政[1] 唐朝枢[1] 

机构地区：[1]北京大学第一医院心血管研究所,北京100034 [2]北京大学第三医院呼吸科,北京100083 [3]中国人民解放军总医院,北京100853

出　　处：《中国病理生理杂志》2002年第8期889-891,I002,共4页Chinese Journal of Pathophysiology

基　　金："973"国家重大基础研究发展规划资助项目 (No.G2 0 0 0 0 5690 5);国家自然科学基金资助课题 (No 39730 2 2 0 )

摘　　要：目的 :观察钙调神经磷酸酶 (CaN)在大鼠不同器官组织中的活性和蛋白分布。方法 :取正常大鼠不同器官组织 ,应用Westernblot及免疫组织化学方法测定CaN催化亚单位α亚型 (CnAα)在各组织中的蛋白含量及分布 ,并用 [32 P]标记的底物肽测定各组织的CaN活性。结果 :①Westernblot结果显示 ,CnAα在大鼠脑组织中有丰富表达 ,心脏、骨骼肌和肺中亦有表达 ,但肾脏及主动脉未检测出有CnAα表达。②免疫组织化学显示 ,大鼠脑组织、心肌细胞胞浆内、肺内巨噬细胞及细支气管周围的结缔组织、主动脉外膜、肾小血管周围结缔组织及肾远曲小管及集合管的外壁中存在免疫反应阳性产物。③CaN活性在脑组织最高 ,其次为骨骼肌、心肌和肺组织 ,主动脉和肾脏最低。结论 :钙调神经磷酸酶在体内具有广泛分布 。AIM: To investigate the activity and distribution of calcineurin (CaN) in different tissues of rat. METHODS: Using western blot and immunohistochemical staining methods to measure the amount and location of CnAα, isoform of catalytic subunit of CaN in different tissues of rat. CaN activity was measured by labelled substrate peptide. RESULTS: 1. Western blot showed that CnAα expression was detected in brain, heart, skeletal muscle and lung tissues. There was no detectable CnAα expression in kidney and aorta. 2. In immunohistochemical staining study, there was strong immunostaining of CnAα in brain. CnAα was located in cytoplasm of cardiac cell, macrophage and connective tissue of peribronchiolar in lung tissue, aorta adventitia, connective tissue around small vessels and outer wall of renal tube. 3. CaN activity was highest in brain, the following was skeletal muscle, myocardium and lung tissue. CaN activity was lowest in aorta and kidney tissue. CONCLUSION: CaN is widely distributed in rats and might be involved in functional regulation of various organs and tissues.

关 键 词：钙调神经磷酸酶 蛋白印迹法 免疫组织化学 脑 心脏 肺 肾 血管 骨骼 

分 类 号：R33[医药卫生—人体生理学] Q55[医药卫生—基础医学]