MLR中刺激细胞活化状态对应答T细胞CD69表达的影响  

Effect of activation of stimulator cells on expression of CD69 by responder T cells in mixed lymphocyte reaction

作　　者：肇静娴[1] 曾耀英[1] 何贤辉[1] 狄静芳[1] 曾山[1] 王南[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广东广州510632

出　　处：《中国病理生理杂志》2002年第8期913-917,共5页Chinese Journal of Pathophysiology

基　　金："973"二级项目 (No .G1 9990 5430 3);国家自然科学基金项目 (No .39670 690 )

摘　　要：目的 :研究混合淋巴细胞反应 (MLR)中刺激细胞的活化状态对应答T细胞活化的影响 ,为指导临床的组织器官移植提供一定理论依据。方法 :混合淋巴细胞培养 (MLC)前对刺激细胞进行不同预处理以改变其功能状态 ,利用单克隆抗体与流式细胞术 ,在混合培养后不同时点对各组应答T细胞活化表面分子CD69的表达程度进行分析。结果 :在培养 2 4h、48h和 72h后 ,MLCa组 (刺激细胞经预先活化组 )应答T细胞的CD69表达百分率均明显高于MLC组 (刺激细胞未经预先活化组 ) ,分别为 5 2 1 %± 0 2 4 %vs 1 98%± 0 33 % ,2 9 81 %± 0 85 %vs 2 0 65 %±1 0 0 %和 39 61 %± 1 62 %vs 1 3 49%± 0 60 % ,(P <0 0 1 )。结论AIM: To study the influence of status of stimulator cells on activation of responder T cells in mixed lymphocyte reaction (MLR), so as to provide some basis for clinical transplantation. METHODS: Stimulator cells were pretreated differently before mixed lymphocyte culture (MLC) to change their functional status, fluorescence conjugated antibodies and flow cytometry were used to detect expression of CD69 by responder T cells at several different time points. RESULTS: The expression percentages of CD69 by responder T cells in MLCa group (stimulator cells were pre-activated) were significantly higher than those in MLC group (stimulator cells were not pre-activated) at 24, 48 and 72 hours of culture, respectively (5 21%±0 24% vs 1 98%±0 33%, 29 81%±0 85% vs 20 65%±1 00% and 39 61%±1 62% vs 13 49%±0 60%, P <0 01). CONCLUSION: Our results showed that with pre-activated stimulator cells, expression of CD69 by responder T cells could be significantly elevated in MLR.

关 键 词：刺激细胞 T细胞 混合淋巴细胞培养试验 活化分析 流式细胞术 器官移植 移植免疫 

分 类 号：R392.4[医药卫生—免疫学]