孤束核参与室旁核加压素能神经元对大鼠胃缺血-再灌注损伤的调控作用  

Nucleus tractus solitarius participates in regulation of paraventricular nucleus vasopressinergic neurons on gastric ischemia-reperfusion injury in rats

作　　者：张咏梅[1] 张建福[1] 阎长栋[1] 周秀萍[1] 祁友键[1] 

机构地区：[1]徐州医学院生理学教研室,江苏徐州221002

出　　处：《中国病理生理杂志》2002年第8期923-926,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .39970 2 81 );江苏省教育厅自然科学基金资助项目 (No .99KJB31 0 0 0 5)

摘　　要：目的 :探讨孤束核在下丘脑室旁核 (PVN)加压素能神经元对大鼠胃缺血 -再灌注损伤 (GI-RI)调控中的作用。方法 :复制夹闭大鼠腹腔动脉 30min ,松开动脉夹血流复灌 1h的GI-RI模型 ,观察核团内微量注射、电刺激、损毁等对其影响。结果 :PVN内注射精氨酸加压素 (AVP)能明显减轻GI-RI,且具有剂量 -效应依赖关系 (r=- 0 477,P <0 0 5) ;损毁双侧孤束核 (NTS)或NTS内给予AVP受体阻断剂均能取消电刺激PVN对GI-RI的减轻作用 ;NTS内注射AVP的作用与PVN内注射AVP的效应相似。结论 :孤束核参与下丘脑室旁核加压素神经元对大鼠胃缺血 -再灌注损伤的调控作用 。AIM: To investigate the role of the nucleus tractus solitarius (NTS) in the regulation of paraventricular nucleus (PVN) AVP-ergic neurons on gastric ischemia- reperfusion injury (GI-RI). METHODS: Male SD rats were used in experiments. The celiac artery were clamped for 30 min and reperfused 1 h by removal of the clamp to obtain the ischemia-reperfusion state. The mechanism was analysed with nucleus electrical stimulation, electrolytic lesion and nucleus microinjection technique. RESULTS: Microinjection of arginine-vasopressin (AVP) into PVN obviously attenuated the GI-RI and dose-dependent effects were observed ( r= -0.477, P< 0.05) ; NTS lesion or microinjection of AVP antagonist into NTS both eliminated the attenuate effect of electrical stimulation of PVN on GI-RI. The effect of microinjection of AVP into NTS was similar to microinjection of AVP into PVN. CONCLUSION: The NTS participates in regulation of PVN AVP-ergic on GI-RI, which is mediated by the AVP receptor in the NTS.

关 键 词：孤束核 胃缺血-再灌注损伤 下丘脑室旁核 精氨酸升压素 

分 类 号：R656.6[医药卫生—外科学]