去甲肾上腺素和缺血预处理对大鼠缺血再灌注心肌细胞凋亡、Bcl-2、Bax蛋白表达的影响  

Effects of norepinephrine preconditioning and ischemic preconditioning on apoptosis and Bcl-2, Bax expression in rat myocardial cells during myocardial ischemic reperfusion

作　　者：程芳洲[1] 唐国华[2] 李庚山[1] 余细球[3] 李远重[2] 贺新华 鲍翠玉[2] 周云[2] 

机构地区：[1]武汉大学人民医院心内科,湖北武汉430060 [2]咸宁医学院附属第一医院内科,湖北咸宁437100 [3]武汉大学中南医院消化科,湖北武汉430071

出　　处：《中国病理生理杂志》2002年第8期949-952,共4页Chinese Journal of Pathophysiology

基　　金：湖北省教育厅 2 0 0 0年科研基金资助项目(No.2 0 0 0B440 0 9)

摘　　要：目的 :研究去甲肾上腺素预处理 (NE -P)和缺血预处理 (IP)对大鼠缺血再灌注 (I/R)心肌细胞凋亡及相关基因Bcl- 2和Bax蛋白表达的影响。方法 :复制缺血再灌注损伤 (IRI) ,采用末端标记技术 (TUNEL)检测心肌细胞凋亡 ;应用免疫组化SABC法检测Bcl- 2和Bax蛋白表达。结果 :I/R组凋亡细胞较多 ,NE -P组及IP组凋亡细胞明显少于I/R组 (P <0 .0 1 )。在I/R组Bcl- 2的表达少而Bax的表达较多 ,NE -P组及IP组Bcl- 2的表达明显高于I/R组 (P <0 .0 1 ) ,而Bax的表达明显低于I/R组 (P <0 .0 1 )。NE -P组与IP组各指标均无显著差异 (P >0 .0 5)。结论 :NE -P可抑制I/R诱发的心肌细胞凋亡 ,Bcl- 2和Bax的蛋白表达在心肌凋亡的发生中起重要作用。NEAIM: To study the effects of norepinephrine preconditioning(NE-P) and ischemic preconditioning (IP)on apoptosis and Bcl-2, Bax expression in rat myocardial cells in myocardial ischemic reperfusion (I/R). METHODS: The model of rat ischemic-reperfusion was used to conduct NE-preconditioning. Apoptotic myocytes were detected with TUNEL. Bcl-2, Bax expression were detected with immunohistochemistry. RESULTS: The rate of apoptosis cells in I/R group was higher, the rate of apoptosis cells in NE-P group and IP was lower significantly than that in I/R group( P< 0.01). The expression of Bcl-2 in I/R group was lower, but the expression of Bax was higher, the expression of Bcl-2 in NE-P group was higher significantly than that in I/R group( P< 0.01), the expression of Bax in NE-P group was lower than that in I/R group( P< 0.01). There was no significantly difference between NE-P and IP group in the above parameters ( P >0.05). CONCLUSION: NE-P reduced myocyte apoptosis by I/R in rats; The expression of Bcl-2 ,Bax genes played an important role in myocardial apoptosis.

关 键 词：去甲肾上腺素 缺血预处理 细胞凋亡 BCL-2基因 BAX基因 心肌缺血再灌注 

分 类 号：R654.2[医药卫生—外科学]