人脐血CD34^+细胞急性期移植对脊髓损伤的修复作用  

Repairing effect of human umbilical cord blood CD34^+ cells transplantation in the acute phase on spinal cord injury

作　　者：唐亮[1] 冯世庆[2] 高瑞霄[3] 

机构地区：[1]天津市海河医院骨科,300350 [2]天津市海河医院内科,300350 [3]天津医科大学总医院骨科,天津300052

出　　处：《广东医学》2015年第18期2783-2787,共5页Guangdong Medical Journal

基　　金：天津市科技计划项目(编号:08ZCKFSF03200)

摘　　要：目的探讨人脐血CD34+细胞急性期移植修复脊髓损伤的效果和血管修复机制。方法免疫磁珠法从人新鲜脐血中分离得到CD34+细胞。雌性Wistar大鼠96只,以IMPACTOR MODEL-Ⅱ脊髓损伤打击器建立T10脊髓损伤模型,随机平均分成A、B、C 3组:A组造模后给予PBS局部移植;B组造模后给予环孢霉素A灌胃联合地塞米松静脉注入治疗,C组造模后给予细胞局部移植及环孢霉素A灌胃联合地塞米松静脉注入治疗。对各组后肢功能恢复情况进行BBB评分,损伤中心行双重免疫荧光染色、2,3,5-氯化三苯基四氮唑(TTC)染色和血管明胶墨汁灌注观察。结果各组大鼠脊髓损伤后后肢功能均逐渐改善,C组存活移植细胞虽未观察到神经分化,但BBB评分均高于其余两组(P<0.01),TTC染色显示组织活力降低区域比例小于其余两组(P<0.01),血管明胶墨汁灌注血管密度大于其余两组(P<0.01)。组织活力的恢复、损伤中心血管密度的增大和后肢功能的改善具有一致性变化。结论人脐血CD34+细胞可能通过提高脊髓损伤中心血管密度促进微循环恢复,增加组织活力,促进大鼠脊髓损伤后肢体功能恢复。Objective To observe the efficacy of human umbilical cord blood CD34＋cells transplantation on spinal cord injury and to investigate the mechanism of vascular repair.Methods CD34＋cells were separated from fresh human umbilical cord blood by magnetic-activated cell sorting.96 female Wistar rats were injured at T10 by IMPACTOR MODEL-Ⅱ,then randomly assigned to 3 groups：Group A,local injection of PBS into the injured epicenter of the spinal cord; Group B,intragastric administration of cyclosporin A and intravenous infusion of dexamethasone; Group C,local transplantation of cells into the injured epicenter of the spinal cord combined with intragastric administration of cyclosporin A and intravenous infusion of dexamethasone.Recovery of the lower limbs were assessed using BBB locomotor scoring system.The survival and neural differentiation of transplanted cells at the injury site were observed by double immunofluorescence.Tissue vitality at the injury site was observed by 2,3,5-triphenyl-2H-tetrazolium chloride staining and the blood vessel density at the injury site was observed by infusing mixture of Chinese ink and glutin followed by HE staining.Results BBB score increased gradually in all groups.Transplanted cells could survive,but neural differentiation was not observed.Nevertheless,BBB score was higher in Group C than in the other groups（ P 0.01）.Percentage of the area with decreased vitality in Group C was smaller than the other groups（ P 0.01）.The blood vessel density in Group C was higher than the other groups（ P 0.01）.Recovery of the lower limbs was consistent with recovery of tissue vitality and increase of the blood vessel density.Conclusion Human umbilical cord blood CD34＋cells can promote recovery of the lower limbs after spinal cord injury by repairing blood vessels and restoring microcirculation to increase tissue vitality at the injury site in rats.

关 键 词：脊髓损伤 人脐血CD34+细胞 组织活力 血管密度 神经分化 

分 类 号：R392.12[医药卫生—免疫学]