解热醒神注射液对内毒素性发热家兔下丘脑cAMP、IL-1_β含量的影响  

Effect of Jiere Xingshen Injection on cAMP, IL-1_β content in hypothalamus of ET febrile rabbits

作　　者：张丹卉[1] 蒋玉凤[1] 黄启福[1] 贾旭[1] 严京[1] 路雪雅[2] 

机构地区：[1]北京中医药大学病理教研室,北京100029 [2]北京中医药大学生化研究室,北京100029

出　　处：《中国病理生理杂志》2002年第8期956-958,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :观察解热醒神 (JRXS)注射液对内毒素 (ET)性发热 1h家兔体温、下丘脑cAMP、IL - 1 β 含量的影响。方法 :复制家兔ET性发热模型 ,用放免法检测下丘脑及脑脊液cAMP、下丘脑IL - 1 β 的含量。结果 :ET组的ΔT[(0 .40± 0 1 1 )℃ ]、TRI1 (1 78± 0 79)、下丘脑cAMP含量 [(2 90± 0 40 )nmol/g]、脑脊液cAMP含量 [(32 1 0± 4 51 )nmol/L) ]、下丘脑IL - 1 β 含量 [(6 0 8± 0 79)ng/g]显著高于生理盐水 (NS)组及JRXS +ET组 (P <0 0 1 )。JRXS +ET组ΔT[(0 1 0± 0 1 0 )℃ ]、TRI1 (0 36± 0 64)、下丘脑cAMP含量 [(1 37± 0 2 7)nmol/g]、脑脊液cAMP含量 [(1 4 40±3 69)nmol/L) ]、下丘脑IL - 1 β 含量 [(2 90± 0 37)ng/g]接近NS组 ,但与ET组比较有非常显著差异 (P <0 0 1 ) ;4组下丘脑及脑脊液cAMP含量、下丘脑IL - 1 β 含量变化与体温变化呈正相关。结论 :JRXS能明显抑制ET引起的发热反应 ,其抑制效应的机制与降低ET引起的下丘脑cAMP、IL - 1 βAIM: The effects of Jiere Xingshen(JRXS) Injection on cAMP, IL-1 β content in hypothalamus (HP) of endotoxin(ET)-induced feverish rabbits were studied. METHODS: The ET-induced fever model was established in rabbits and the cAMP content in hypothalamus (HP) and csf, IL-1 β content in HP were determined by radioimmunoassay following intravenous infusion of JRXS. RESULTS: In ET group, the ΔT [(0 40±0 11)℃], TRI 1(1 78±0 79), cAMP content in HP [(2 90±0 40) nmol/g], cAMP content in csf [(32 10±4 51) nmol/L)], IL-1 β content in HP[(6 08±0 79) ng/g] were higher than that of NS and JRXS+ET group ( P <0 01) In JRXS+ET group, the ΔT[(0 10±0 10)℃], TRI 1(0 36±0 64), cAMP content in HP [(1 37±0 27) nmol/g], cAMP content in csf [(14 4±3 69) nmol/L)], IL-1 β content in HP [(2 90±0 37)ng/g] were very close to that of NS group but lower than that of the ET group ( P< 0 01);The cAMP content in HP and csf, IL-1 β content in HP paralleled with the fluctuation of temperature. CONCLUTION: JRXS Injection has significant inhibitory effect on ET-induced fever by inhibiting cAMP and IL-1 β production in hypothalamus.

关 键 词：解热醒神注射液 内毒素性发热 下丘脑 CAMP 白细胞介素-1 

分 类 号：R255.1[医药卫生—中医内科学] R285[医药卫生—中医学]