吗啡依赖大鼠脊髓和脑干毒蕈碱受体亚型基因的表达  

mRNA EXPRESSION OF MUSCARINIC RECEPTORS IN SPINAL CORD AND BRAINSTEM IN MORPHINE DEPENDENT RATS

作　　者：周文华[1] 刘惠芬[1] 顾钧[1] 谢小虎[1] 唐甩恩[1] 杨国栋[1] 吴其夏[2] 

机构地区：[1]宁波微循环与莨菪类药研究所宁波戒毒研究中心,浙江宁波315010 [2]中国协和医科大学病理生理教研室,北京100050

出　　处：《药学学报》2002年第8期611-615,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目 (3 0 10 0 0 5 1)

摘　　要：目的　观察吗啡依赖大鼠脊髓和脑干毒蕈碱型乙酰胆碱受体m1～ 5的表达。方法　以 β actin为内标 ,用RT PCR方法检测m1～ 5的表达。结果　吗啡依赖大鼠脊髓m1～ 5受体和脑干中m1 和m2 表达较正常对照组明显升高 ,注射纳洛酮 1h后脊髓m1～ 4 和脑干m1 表达较依赖组减少。东莨菪碱 (0 5mg·kg- 1 ,ip)或呱伦西平 (10mg·kg- 1 ,ip)明显减少吗啡戒断症状 ,呱伦西平处理后脊髓m1 ,m2 ,m3和m5表达较戒断对照组增加 ,东莨菪碱处理后脊髓m2 ,m3和m4 表达增加。结论　脊髓M受体适应性改变是吗啡戒断症状表达的生物学基础。AIM To observe mRNA expression of muscarinic acetylcholine receptors in spinal cord and brainstem in morphine dependent or withdrawal rats. METHODS The mRNA expression level of m 1, m 2, m 3, m 4 and m 5 were determined by RT PCR, the β actin mRNA expression was used as internal control. RESULTS The mRNA level of m 1, m 2, m 3, m 4 and m 5 in spinal cord and m 1 and m 2 in brainstem were increased significantly during morphine dependence, and the levels of m 1, m 2, m 3 and m 4 in spinal cord and m 1 in brainstem were decreased 1 h after the injection of naloxone (4 mg·kg -1 , ip) in morphine dependent rats. Either scopolamine (0 5 mg·kg -1 ) or pirenzepine (10 mg·kg -1 ) was shown to significantly decrease the morphine withdrawal symptoms in rats. The levels of m 1, m 2, m 3 and m 5 in spinal cord were increased by pretreatment with pirenzepine and the levels of m 2, m 3 and m 4 in spinal cord were increased by pretreatment with scopolamine. CONCLUSION The adaptive expression of muscarinic receptors at spinal and supraspinal levels play important role in mediating morphine dependence and withdrawal in rats.

关 键 词：毒蕈碱受体 吗啡依赖 东莨菪碱 阿片受体 戒断症状 基因表达 

分 类 号：R595.5[医药卫生—内科学]