苦豆子不同提取部位抗HepG2活性的研究  

Study on the antitumor activity of different extracted parts of Sophora Alopecuroides

作　　者：蒲秀瑛[1] 刘璐[1] 马小龙[1] 任菁[1] 李晓玥[1] 李海兵[1] 

机构地区：[1]兰州理工大学生命科学与工程学院甘肃省中藏药筛选评价及深加工重点实验室,甘肃兰州730050

出　　处：《中国食品工业》2015年第8期60-61,共2页China Food Industry

基　　金：自然科学基金项目（81260070）

摘　　要：研究苦豆子不同提取部位对肝癌细胞H印G2的抑制作用。方法：根据溶剂极性的大小对苦豆子进行提取，以细胞存活率和IC50为指标，采用噻唑蓝（MTT）比色法检测苦豆子不同提取部位体外对肝癌细胞HepG2的抑制作用。结果：苦豆子的乙醇部位和正丁醇部位在实验浓度范围（0．125-1mg／mL）内对HepG2细胞的抑制作用较强，其IC50分别是：O．21mg／mLSUO．92mg／mL，石油醚部位在实验浓度范围内对HepG2细胞未显示出抑制作用。结论：苦豆子乙醇部位和正丁醇部位对HepG2细胞有明显的抑制作用。Objective： To investigate different extracted parts of sophora alopecuroides anti-HepG2 activity in vitro. Methods： The sophora alopecuroides were extracted by different polarity solvents. MTT assay was used to observe the inhibitory effect of different extracted parts of sophora alopecuroides on liver cancer cell HepG2 in vitro. Ethanol extraction and N-butyl alcohol extraction of sophora alopecuroides had the cell inhibition activity against HepG2 （0.125-1 mg/mL）,the IC50 were 0.21 mg/mL and 0.92 mg/mL, petroleum ether extraction of sophora alopecuroides showed no inhibition on HepG2. Results： the sophora alopecuroides ethanol part and n-butanol part has the obvious inhibitory effect on HepG2 .

关 键 词：苦豆子 不同提取部位 丝裂霉素 HEPG2 

分 类 号：X835[环境科学与工程—环境工程]