玉米光合性状的相关性及QTL分析  

Correlation and QTL Analyses for Photosynthetic Traits in Maize

作　　者：余婷婷[1] 刘朝显[1] 梅秀鹏 王久光[1] 王国强[1] 蔡一林[1] 

机构地区：[1]西南大学农学与生物科技学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2015年第9期1-10,共10页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市重大攻关项目(CSTC2012ggc80003;CSTC2012ggc80006;CSTC2012ggc80007)资助

摘　　要：为了探讨玉米光合性状的相关性及遗传机制,利用2个不同遗传背景的F2群体对叶绿素a质量分数、叶绿素b质量分数、叶绿素总质量分数、净光合速率、气孔导度、胞间CO2浓度和蒸腾速率等10个光合性状进行了相关性及QTL分析.在相关性分析中,对于Y群体,叶绿素质量分数a、叶绿素质量分数b和叶绿素总质量分数3个性状在不同时期的相关性不显著;而对于R群体,这3个性状在不同时期的相关性极显著,达到高度相关.对于其余性状间的相关,两群体表现一致性高.同一时期的叶绿素a质量分数、叶绿素b质量分数和叶绿素总质量分数间为高度相关,净光合速率与气孔导度的相关、净光合速率与蒸腾速率的相关、气孔导度与蒸腾速率的相关为高度相关,气孔导度与胞间CO2浓度的相关为中度相关,叶绿素质量分数与净光合速率、气孔导度、胞间CO2浓度、蒸腾速率间均为弱度相关.在QTL分析中,对于Y群体,五叶期检测到控制叶绿素a质量分数、叶绿素b质量分数、叶绿素总质量分数的QTL各1个,位于第4染色体的umc2391-mmc0371之间,单个QTL可解释表型变异的8.65%~9.87%;乳熟期检测到控制叶绿素a质量分数、叶绿素b质量分数、叶绿素总质量分数的QTL各1个,位于第10染色体的mmc0501-bnlg1451之间,单个QTL可解释表型变异的6.77%~6.93%;散粉期检测到1个净光合速率QTL,1个气孔导度QTL,2个胞间CO2浓度QTL,2个蒸腾速率QTL,单个QTL可解释表型变异的5.64%~7.73%.对于R群体,五叶期检测到3个叶绿素a质量分数QTL、2个叶绿素b质量分数QTL、3个叶绿素总质量分数QTL,其中1个控制叶绿素总质量分数的QTLqRFCt-1-2贡献率超过10%,为主效QTL;乳熟期检测到2个叶绿素a质量分数QTL、3个叶绿素b质量分数QTL、2个叶绿素总质量分数QTL;散粉期检测到2个净光合速率QTL,1个气孔导度QTL,1个胞间CO2浓度QTL,1个蒸腾速率QTL,单个QTL可解释表型变异�In order to explore the correlation and genetic mechanism of maize photosynthetic traits,two F2populations（Y and R）with different genetic backgrounds were used to identify correlation and QTLs for10 photosynthetic traits,which included chlorophyll-a content,chlorophyll-b content,total chlorophyll content,net photosynthetic rate,stomata conductance,intercellular CO2 concentration,transpiration rate and some others.The correlation between two of chlorophyll-a content,chlorophyll-b content and total chlorophyll content in different periods was non-significant in population Y,and was highly significant in population R.The correlation between other traits of the two populations was highly consistent.In the same growth period of the plants,total chlorophyll content was in highly significant correlation with chlorophyll-a content and chlorophyll-b content,highly significant correlation was also detected between net photosynthetic rate and stomata conductance,between net photosynthetic rate and transpiration rate,and between stomata conductance and transpiration rate,stomata conductance was moderately correlated with intercellular CO2 concentration,and chlorophyll content was weakly correlated with net photosynthetic rate,stomata conductance,intercellular CO2 concentration and transpiration rate.In population Y,1QTL was detected for chlorophyll-a content（FCa）,chlorophyll-b content（FCb）and total chlorophyll content（FCt）each,at the five-leaf stage.All these QTLs were located in umc2391-mmc0371 on chromosome 4and each explained 8.65%to 9.87% of the phenotypic variance.And other three QTLs were detected at the milk stage,one for chlorophyll-a content,one for chlorophyll-b content and one for total chlorophyll content.They were located in mmc0501-bnlg1451 on chromosome 10 and explained 6.77 to 6.93% of the phenotypic variance each.Six QTLs were detected at the pollination stage,one for net photosynthetic rate,one for stomata conductance,two for intercellular CO2 concentration and two for transpiration rate,and

关 键 词：玉米 叶绿素质量分数 光合性状 相关性 QTL分析 

分 类 号：S513[农业科学—作物学]