天然四倍体红江橙与其二倍体的植株形态学和遗传组成比较  

Morphological Comparison and Genetic Difference Between a Natural Tetraploid and the Diploid of Citrus sinensis cv.Hongjiangcheng

作　　者：王莹[1] 李晓林[1] 梁国鲁[1] 向素琼[1] 

机构地区：[1]西南大学园艺园林学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2015年第9期37-41,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金(31272138);国家科技支撑计划(2013BAD02B02);中央高校基本科研业务费专项资金(XDJK2012B019)资助

摘　　要：对天然四倍体红江橙与其二倍体的植株形态学和基因组遗传组成进行了比较.天然四倍体红江橙植株表现出与二倍体明显不同的植株形态特征,尤其是:生长较缓慢,树冠较小,树高与干周为二倍体的0.89和0.83倍;春梢长度仅为二倍体的0.7倍,春梢成熟叶片叶形指数减小,为二倍体的0.87倍,叶片厚度增加明显,为二倍体的1.36倍、对天然红江橙四倍体基因组遗传组成的GISH与SSR分析显示,天然四倍体红江橙是由二倍体加倍而来的同源四倍体,但又与二倍体基因组的遗传组成存在DNA水平上的变异.A comparison was made of the morphological characteristics and the genetic composition of the genome between the diploid and natural tetraploid of＂Hongjiangcheng＂.Morphologically,the tetraploid was shown to be strikingly different from the diploid plants.It had poorer growth rate and smaller tree crown.The height and circumference of its trunk was 0.89 and 0.90 times those of the diploid,respectively.The length of vernal shoots of the tetraploid was 0.7times that of the diploid.The leaves of the tetraploid were broader and obviously thicker than those of the diploid.The leaf index and the leaf thickness of the tetraploid were 0.87 and 1.36 times those of the diploid.GISH（genome in situ hybridization）and SSR analyses of the genetic copmsotion of the genome showed that the teraploid plant was an autotetraploid,resulting from chrmosomome doubling of the female diploid and that some DNA variations occurred between the natural tetraploid and the diploid.

关 键 词：红江橙 四倍体 基因组原位杂交(GISH) 简单重复序列(SSR) 

分 类 号：S666[农业科学—果树学]