电针对SAMP8小鼠行为学及APP mRNA和BACE-1 mRNA影响  

Effects of electroacupuncture on expression of APP mRNA and BACE-1m RNA in SAMP8 mice

作　　者：郎尉雅[1] 张海燕[1] 刘忠锦[2] 

机构地区：[1]齐齐哈尔医学院组织学与胚胎学教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院第一附属医院神经内科,黑龙江齐齐哈尔161041

出　　处：《中国临床解剖学杂志》2015年第5期563-567,共5页Chinese Journal of Clinical Anatomy

基　　金：黑龙江省教育厅科学技术研究资助项目(12521652)

摘　　要：目的探讨电针对SAMP8小鼠行为学及淀粉样前体蛋白(amyloid precursor protein,APP)和APP切割酶1(B site APP cleaving enzyme1,BACE-1)的影响,为临床治疗阿尔茨海默病病提供新的途径。方法将快速老化模型小鼠(SAMP8)60只随机分成模型组、电针组和西药组,每组20只,正常老化小鼠(SAMR1)20只作为正常组。电针组电针刺激"百会"、"肾俞"、"内关"、"大椎"穴;西药物组给予石杉碱甲0.02 mg/kg剂量灌胃;正常组和模型组不给予治疗。治疗20 d后,用Morris水迷宫检测小鼠学习记忆能力,免疫组化定性检测小鼠海马APP和BACE-1蛋白的表达,进一步实时荧光定量PCR法测定APP mRNA和BACE-1 mRNA的表达。结果与模型组比较,西药组与电针组逃避潜伏期缩短,有效区停留时间和及跨越平台次数均显著增加(P<0.05),电针组APP mRNA和BACE-1 mRNA相对表达量显著减少(P<0.05)。结论电针可能抑制SAMP8小鼠脑内APP mRNA and BACE-1 mRNA表达,减少Aβ生成,改善其学习记忆障碍。Objective To explore the effects of electroacupuncture on behavior and APP mRNA and BACE-1 mRNA in senescence accelerated mouse （SAMP8）. This provides a further evidence for therapy of Alzheimer＇s disease. Methods Sixty senescence accelerated mice prone 8 （SAMP8） were randomized into a model group,an electroacupuncture group and a medicine group. Twenty mice of senescence accelerated mouse/ resistance 1（SAMP1） were in a normal group. In the electroacpuncture group,electroacupuncture was applied to＂Baihui＂,＂Shenyu＂,＂Neiguan＂and＂Dazhui＂.In the medicine group,the gastric infusion with Huperzine A was applied 0.02 mg/kg. The normal group and model group were not treated. After treatment for 20 days,Morris water maze test was used to determine the learning and memory ability of mice. The expression of APP and BACE-1 was determined with immunohistochemistry. The real-time fluorescent quantitative PCR was applied to determine the expression of APP mRNA and BACE-1 mRNA. Results Compared with the model group,the escaping latent period was reduced significantly in the medicine group and the electroacupuncture group,frequencies of leaping and the target quadrant swimming time was increased significantly（P〈0.05）; the expressions of APP mRNA and BACE- 1 mRNA were reduced significantly in the electroacupuncture group（P〈0.05）. Conclusion Electroacupuncture has improvements in learning and memory disturbance probably through inhibiting APP mRNA and BACE-1 mRNA expression and reduced cerebral Aβlevel in SAMP8 mice.

关 键 词：阿尔茨海默病 淀粉样前体蛋白 APP切割酶1 

分 类 号：Q3[生物学—遗传学]