Wistar大鼠口腔颌面部微血管铸型制作  

Researching microvascular casting model of oral and maxillofacial regions of Wistar rats

作　　者：毕振宇[1] 刘阳 孙培栋[1] 杨宇超 欧阳钧[1] 

机构地区：[1]南方医科大学人体解剖学教研室广东省医学生物力学重点实验室,广州510515 [2]深圳市龙华新区人民医院口腔科,深圳518109

出　　处：《中国临床解剖学杂志》2015年第5期604-607,共4页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(31170903)

摘　　要：目的通过Wistar大鼠头面部微血管铸型标本制作与扫描电镜观察,旨在建立毛细血管真实的三维空间显示方法,以便于毛细血管三维数字化重建。方法 8周龄Wistar大鼠从腹腔以下断除,从主动脉插管冲洗后,灌注树脂材料。恒温水浴箱放置24 h后,解剖大鼠头颈部,形成皮肤标本、舌标本、下颌骨标本、上颌骨标本。将标本腐蚀、冲洗、干燥。用离子镀膜机进行喷镀金,在扫描电镜下观察血管铸型的解剖形态、走形、以及毗邻结构。结果成功制备了Wistar大鼠的皮肤标本、舌标本、下颌骨标本、上颌骨标本的毛细血管铸型,清晰显示局部毛细血管的形态与走形结构,扫描电镜下可显示微观毛细血管三维空间结构。结论建立了Wistar大鼠头面部的毛细血管铸型方法,为研究微血管的形态学三维重建提供了方法,也为毛细血管的3D打印奠定了基础。Objectives To build the capillaries casting method of Wistar rats maxillofacial microvascular specimens by perfusion combined with scanning electron microscopy （SEM）, so that the three-dimensional digital models of capillaries were established. Methods Maxillofacial microvascular specimens of 8 weeks of Wistar rats were resected from the abdominal cavity. The catheter was inserted from ventricle to the aorta. The resin material was filled, and specimen was placed in constant temperature water bath for 24 hours. The sample was divided into some parts, such as skin, tongue, jaw bones. The samples were corroded, washed, dried, and plated gold. The anatomical morphology of vascular casting was observed under scanning electron microscopy, course and adjacent structures. Results Capillary casting specimens of skin, tongue, mandible and maxilla of Wistar rats were successfully prepared. They clearly showed the morphology and course of the capillaries in the specimens. Under the scanning electron microscopy, the micro capillary structures of the capillaries were showed in three dimensions. Conclusion The facial capillaries casting methods in Wistar rats can serve as the basis for 3D digital model of the microvascular morphology and 3D printing of the capillaries.

关 键 词：微循环 WISTAR大鼠 微血管铸型 模型 

分 类 号：R323.1[医药卫生—人体解剖和组织胚胎学]