北沙参内生真菌多样性研究  

Diversity of endophytic fungi in Glehnia littoralis

作　　者：侯晓强[1,2] 任秀艳[1,2] 付亚娟[1,2] 乔洁[1,2] 张志丽[1] 吕寒聪 

机构地区：[1]廊坊师范学院生命科学学院,河北廊坊065000 [2]河北省高校食药用菌应用技术研发中心,河北廊坊065000

出　　处：《广东农业科学》2015年第18期20-24,共5页Guangdong Agricultural Sciences

基　　金：河北省教育厅科研项目(2011108;QN2014129);廊坊师范学院微生物学重点学科项目(2015001)

摘　　要：对北沙参内生真菌类群组成及多样性进行分析。结果表明,从北沙参植株中分离到内生真菌137株,利用ITS-r DNA序列分子鉴定法将北沙参生真菌鉴定为21个分类单元,分布在6纲8目11科21属。河北安国北沙参内生真菌归于10个属,内生真菌的多样性指数H=1.08、D=0.90,优势菌群为链格孢属(Alternaria)真菌,占总菌株数的29.93%;山东烟台北沙参内生真菌归于17个属,内生真菌的多样性指数H=1.31、D=0.94,以刺盘孢属(Colletotrichum)和链格孢属(Alternaria)真菌为优势菌群,分别占总菌株数的19.71%和13.87%,两地北沙参内生真菌种类的相似性系数Cs=0.57。由此可见,两地北沙参内生真菌组成具有一定的相似性,山东烟台北沙参内生真菌多样性高于河北安国北沙参。The endophytic fungi were isolated from Glehnia littoralis. The molecular identification of ITSr DNA was applied to analyze fungal taxa,and fungal diversity was explored. The results showed that 137 fungal strains were isolated from G. littoralis. These fungi were assigned to 21 taxa,containing 6 classes,8 orders,11 families and 21 generas. Endophytic fungal groups in G. littoralis of Anguo were attributed to 12 generas. ShannonWiener index（H）and Simpson’s index（D）were 1.08 and 0.90 respectively. Alternaria（29.93%）was the major fungal group. Endophytic fungal groups in G. littoralis of Yantai were attributed to 17 generas. The diversity index were 1.31（H）and 0.94（D）respectively. Colletotrichum（19.71%）and Alternaria（13.87%）belonged to the core groups. Thus,the diversity of endophytic fungi in Yantai G. littoralis was higher than that of Anguo G. littoralis. Moreover,there was a medium similarity（Cs=0.57）of endophytic fungal composition in G. littoralisbetween Anguo and Yantai.

关 键 词：北沙参 内生真菌 分离 鉴定 多样性 

分 类 号：Q939.5[生物学—微生物学]