丹参对兔骨性关节炎关节软骨白介素-1β、白介素-6和胰岛素样生长因子-Ⅰ表达的影响  

Effects of danshen on expressions of interleukin-1β,interleukin-6 and insulin like growth factor-Ⅰ in articular cartilage of rabbits with osteoarthritis

作　　者：白斌[1] 李燕琴[2] 李曙明[1] 赵为公[1] 王坤正[3] 

机构地区：[1]西安交通大学第一附属医院骨科,西安710061 [2]西安交通大学医学部公共卫生学院流行病与卫生统计学系 [3]西安交通大学第二附属医院骨科

出　　处：《中国康复医学杂志》2015年第10期990-994,共5页Chinese Journal of Rehabilitation Medicine

基　　金：陕西省科技攻关项目(2008K14-01)

摘　　要：目的:探讨丹参对兔骨性关节炎(OA)模型关节软骨退变的保护作用及其机制。方法:将24只大白兔随机分成2组,即对照组和实验组,每组12只。所有兔通过手术建立右膝关节OA动物模型,对照组的所有兔的右膝关节腔内注射生理盐水0.5ml/kg,实验组所有兔的右膝关节腔内注射丹参注射液0.5ml/kg,手术完毕后当天就注射1次,以后每4天注射1次。在动物OA模型建立后的第10周,将兔处死,收集右侧股骨内侧髁标本,用光学显微镜和透射式电子显微镜观察关节软骨形态学结构改变,用免疫组化技术检测关节软骨组织中IL-1β、IL-6和IGF-Ⅰ蛋白的表达。结果:实验组兔的关节软骨破坏程度明显轻于对照组(P<0.05)。IL-1β、IL-6和IGF-Ⅰ蛋白阳性免疫反应物主要定位于细胞浆,为粗细较为一致的淡红色颗粒。实验组IL-1β蛋白阳性染色强度明显低于对照组(P<0.05)。实验组IL-6和IGF-Ⅰ蛋白阳性染色强度明显高于对照组(P<0.05)。结论:丹参能调节OA关节软骨组织中IL-1β、IL-6和IGF-Ⅰ蛋白的异常表达,对关节软骨具有保护作用。Objective： To study the effects of danshen in preventing articular cartilage degeneration of rabbits wltla osteoarthritis（OA）. Method： Twenty-four rabbits were randomly divided into 2 groups, control group and experimental group （n= 12）. The models of OA were established by operation in right knee joints of rabbits. Saline solution （0.5ml, per kg body weight） was infused into the articular cavities of right knee, one times per 4 days in control group. Danshen solution （0.5ml, per kg body weight） was infused into the articular cavities of right knees, one times per 4 days in experimental group. Ten weeks after establishing OA model, all rabbits were sacrificed and the medial condyles of right femurs were collected. The morphological and histological changes of articular cartilages were observed by light microscope and electron microscope. The expressions of interleukin-1β（IL- 1β）, interleukin-6（IL-6） and insulin like growth factor-Ⅰ （IGF-Ⅰ ） were probed by immunohistochemistry tech- nique. Result： The morphological changes of articular cartilage in control group were severer than those in experimental group （P〈0.05）. The IL-1β, IL-6 and IGF-Ⅰ positive staining were red granule and located in cytoplast of cartilage cell. The positive staining intensity of IL-1β in experimental group was lower than that in control group （P〈0.05）. The positive staining intensity of IL-6 and IGF-Ⅰ in experimental group were higher than that in control group（P〈 0.05）. Conclusion： Danshen possesses protective role on early cartilage degeneration by regulate the expressions of IL- 1β, IL-6 and IGF-Ⅰ in articular cartilage of osteoarthritis.

关 键 词：丹参 关节软骨 白细胞介素-1Β 白细胞介素-6 胰岛素样生长因子-Ⅰ 

分 类 号：R285.5[医药卫生—中药学]