运动预处理后内源性阿片肽对大鼠心肌缺血再灌注损伤的延迟性保护作用  

Delayed cardioprotection of endogenous opioid peptides in rats with myocardial ischemic reperfusion injury after exercise preconditioning

作　　者：王强[1] 闻剑飞[1] 汪辉[1] 陆保云 宋旭[1] 

机构地区：[1]合肥师范学院体育科学学院230601

出　　处：《中国康复医学杂志》2015年第10期995-1001,共7页Chinese Journal of Rehabilitation Medicine

基　　金：安徽省高校省级优秀青年人才基金重点项目(2012SQRW126ZD);安徽省高校省级自然科学研究项目(KJ2012Z326)

摘　　要：目的:探讨运动预处理后内源性阿片肽(EOP)对大鼠心肌缺血再灌注损伤延迟性保护作用及机制。方法:健康雄性SD大鼠75只,随机分成假手术组(sham组)、缺血再灌注模型组(I/R组)、运动预处理组(EP组)、运动预处理+纳洛酮(naloxine)组(EPN组)、运动预处理+白屈菜赤碱(chelerythrine)组(EPC)。在运动预适应模型基础上,结扎左冠状动脉前降支复制大鼠心肌缺血再灌注模型,采用多道生理记录仪描记血流动力学参数,用酶联免疫法(ELASA)检测血清心肌肌钙蛋白Ⅰ(cTnⅠ)和心肌环氧化酶-2(COX-2),用化学比色法检测心肌诱导型一氧化氮合酶(i NOS)活性,用黄嘌呤氧化法检测心肌锰超氧化物歧化酶(Mn-SOD)活性,用原子分光光度法检测心肌中钙离子(Ca2+)浓度。结果:1与I/R组相比,EP组在再灌注期左室收缩压(LVSP)、左室内压最大上升速率(+dp/dt max)降低幅度明显减小(P<0.05);与EP组相比,EPC组在再灌注60min后+dp/dt max值下降幅度明显,且差异具有显著性(P<0.05)。2与I/R组相比,EP组血清cTnⅠ明显减少,具有显著性差异(P<0.05);与EP组相比,EPN组和EPC组中血清cTnⅠ值明显升高,有显著性意义(P<0.05)。3与I/R组相比,EP组、EPN组和EPC组心肌中Mn-SOD、i NOS和COX-2活性明显升高,差异有显著性(P<0.05);与EP组相比,EPC组心肌COX-2和i NOS显著降低(P<0.05),EPN组心肌COX-2显著降低(P<0.05)。4与I/R组相比,EP组心肌细胞胞浆中Ca2+浓度明显降低,具有显著性差异(P<0.05),EPN组和EPC组心肌细胞胞浆中Ca2+浓度有降低趋势,但差异不显著;与EP组相比,EPN组和EPC组中心肌细胞胞浆中Ca2+浓度明显升高,有显著性意义(P<0.05)。结论:运动预处理对大鼠心肌缺血再灌注损伤有保护作用,内源性阿片肽可能通过蛋白激酶C(PKC)及下游信号途径减轻心肌细胞钙超载和改善微循环,发挥延迟性保护效应。Objective： To explore the delayed protective effect and mechanism of endogenous opioid peptides （EOP） in rats with myocardial ischemic reperfusion injury after exercise preconditioning. Method： Seventy-five health male SD rats were randomly divided into sham; ischemic reperfusion injury（I/R）, exercise precondition（EP）, exercise precondition ＋naloxone（EPN） and exercise precondition＋chelerythrine（EPC） group. After establishing of EP animal model, the rats were ligated ramus descending coronary artery to induce myocardial ischemic reperfusion. Then the parameter of hemodynamics were traced, and serum cardiac troponin I （cTn l ）, myocardium cyclooxygenase（COX-2） was detected by enzyme linked immunosorbent assay （ELISA）, myocardium iNOS was detected by colorimetric method, myocardium manganese-superoxide dismutase （Mn-SOD） was detected by xanthine hydrocarbonylation and myocardium Ca2＋ density was detected by atom absorption spectrophotometry. Result： （1） As compared with group I/R, reduce range of left ventricular systolic pressure（LVSP）, ±dp/dtmax de- creased significantly in group EP during reperfusion 60min （P 〈 0.05）; As compared with group EP, ±dp/dtmax decreased significantly in group EPC during reperfusion 60min （P〈 0.05）. （2） As compared with group I/R, se- rum cTn [ decreased significantly in group EP （P 〈 0.05）; As compared with group EP, cTn I level in serum increased in group EPN and EPC （P〈 0.05）. （3） As compared with group I/R, Mn-SOD, COX-2, iNOS in myo- cardium increased significantly in group EP, EPN and EPC （P〈 0.05）. As compared with group EP, COX-2, iN- OS in myocardium decreased significantly in group EPC （P〈0.05）, COX-2 in myocardium decreased significantly in group EPN （P〈 0.05）. （4） As compared with group I/R, Ca2＋ level in myocardial sarcoplasmic reticu- lure decreased significantly in group EP （P〈 0.05）, myocardial sarcoplasmic reticulum Ca2＋ decreased without statistical

关 键 词：运动预处理 阿片肽 心肌缺血再灌注 延迟心肌保护 

分 类 号：R285.5[医药卫生—中药学]