胆固醇酯转运蛋白C>T/In9基因多态性与广西人群2型糖尿病亚临床动脉粥样硬化的相关性  

Correlation Between Cholesterol Ester Transfer Protein C > T / In9 Gene Polymorphism and Subclinical Atherosclerosis in Patients With Type 2 Diabetes Mellitus in Guangxi

作　　者：余博先 肖常青[1] 庞翠军[1] 袁雁[1] 宋琳[1] 廖碧芝 

机构地区：[1]广西医科大学第一附属医院内分泌代谢病科,广西南宁市530021

出　　处：《中国全科医学》2015年第29期3582-3586,共5页Chinese General Practice

基　　金：广西自然科学基金资助项目(2011GXNSFA018257);广西中医药管理局基金项目(GZKZ10-113)

摘　　要：目的探讨广西地区人群胆固醇酯转运蛋白(CETP)C>T/In9(rs289714)基因多态性分布特点,及其与糖代谢和2型糖尿病(T2DM)亚临床动脉粥样硬化(SA)的相关性。方法选择2012年12月—2014年6月于广西医科大学第一附属医院内分泌代谢病科住院的T2DM患者83例为T2DM组,均符合1999年WHO推荐的糖尿病诊断及分型标准;选择该院体检中心同期体检健康者68例为对照组。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测两组CETP C>T/In9基因多态性。结果对照组和T2DM组C>T/In9基因型分布及等位基因频率比较,差异均无统计学意义(χ2=0.943、0.004,P>0.05)。T2DM组SA亚组TT、CC、CT基因型分布分别为67.4%、10.9%、21.7%,非SA亚组分别为45.9%、2.7%、51.4%,差异有统计学意义(χ2=8.367,P=0.014)。T2DM组SA亚组与非SA亚组T、C等位基因频率比较,差异无统计学意义(χ2=0.972,P=0.324)。多因素Logistic回归分析显示,CETP C>T/In9基因型和年龄是广西人群T2DM发生SA的影响因素〔OR(95%CI)=0.560(0.317,0.989)、1.126(1.054,1.203),P<0.05〕。结论在广西人群中,暂不能认为CETP C>T/In9基因多态性与糖代谢有关;CETP C>T/In9基因型在T2DM SA和非SA人群中分布不同,CETP C>T/In9基因多态性可能与T2DM SA相关,基因型TT可能是损害性基因型。Objective To investigate the distribution characteristics of cholesterol ester transfer protein （CETP） C 〉 T/In9 （rs289714） gene polymorphism of the population in Guangxi and its correlation with subclinical atherosclerosis （SA） in patients with type 2 diabetes mellitus （T2DM）. Methods We enrolled 83 T2DM patients who were admitted into the Department of Endocrinology and Metabolism of the First Affiliated Hospital of Guangxi Medical University from December 2012 to June 2014 as T2DM group, and the 83 patients all accorded with the criteria of diabetes diagnosis and typing. We also enrolled 68 healthy people who received physical examination in the hospital in the same period as control group. PCR-RFLP was employed to examine the CETP C 〉 T/In9 gene polymorphism. Results Control group and T2DM group were not significantly different in the distribution of C 〉 T/In9 genotypes and allele frequency （x^2 = 0. 943, 0. 004; P 〉 0. 05）. In T2DM group, the proportions ofTr, CC and CT genotypes were 67.4%, 10. 9% and 21.7% for SA subgroup and 45.9%, 2. 7% and 51.4% for non - SA subgroup, with significant differences between the two subgroups （ x^2 = 8. 367, P = 0. 014）. In T2DM group, SA subgroup and non - SA subgroup were not significantly different in T and C allele frequency （ x^2 = 0. 972, P = 0. 324 ）. Multivariate Logistic regression analysis showed that CETP C 〉 T/In9 genotype and age were influencing factors for T2DM patients with SA in Guangxi Province [OR （95% CI） = 0.560 （0.317, 0.989）; 1.126 （1.054, 1.203）, P 〈 0.051. Conclusion For the population of Guangxi, CETP C 〉 T/In9 gene polymorphism could not be considered related with glycometabolism; T2DM patients with SA and those without SA are different in CETP C 〉 T/In9 genotype distribution. CETP C 〉 T/In9 gene polymorphism may be related with SA in T2DM patients, and TT genotype may be the damaging genotype.

关 键 词：胆固醇酯转运蛋白质类 C〉T/In9 糖尿病 2型 亚临床动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病] R587.1[医药卫生—内科学]