消肿止痛合剂对大鼠骨折修复过程中骨痂组织中血管内皮细胞生长因子的影响  

Xiaozhongzhitong Mixture on Rat Fracture Effect of Vascular Endothelial Growth Factor on Bone Callus in the Process of Tissue Repair

作　　者：张雪鹏[1] 杨雨果[2] 

机构地区：[1]南阳理工学院张仲景国医学院,南阳473004 [2]南阳医学高等专科学校,南阳473061

出　　处：《科学技术与工程》2015年第29期105-108,113,共5页Science Technology and Engineering

基　　金：河南省科技厅科技攻关项目(142102310107)资助

摘　　要：研究了消肿止痛合剂对大鼠骨折修复过程中骨痂组织中血管内皮生长因子（VEGF）表达的影响作用。选取健康SD大鼠30只,周龄5~6周,进行编号后采用随机数字表法分为消肿止痛活剂组、模型组各15只,两组SD大鼠制作股骨（单侧）闭合骨折模型,消肿止痛合剂组给予消肿止痛合剂处理（次/12 h）,模型组不予以处理,两组大鼠的健侧股骨作为空白组,比较各组大鼠骨痂组织周围新生血管数量、骨痂组织中VEGF的含量变化情况。第2日、第4日消肿止痛合剂组和模型组大鼠的骨痂组织切片中,未见新生血管形成,第7日,两组大鼠骨痂组织中均有新生血管形成,且第10日数量均较第7日显著增加,第21日两组新生血管数量明显减少;第7日、第10日、第21日消肿止痛活剂组大鼠骨痂组织中新生血管数量均显著的高于模型组大鼠（P〈0.05）。第2日、第4日、第21日消肿止痛合剂组和模型组大鼠的骨痂组织切片中VEGF阳性细胞数量差异无统计学意义（P〉0.05）,第7日,第10日消肿止痛活剂组大鼠骨痂组织中VEGF着色阳性细胞数量均显著高于模型组大鼠（P〈0.05）。消肿止痛合剂有利于促进大鼠骨折修复过程中骨痂组织中新生血管形成、VEGF表达,这可能与促进大鼠骨折愈合有一定的关系。Xiaozhongzhitong mixture on rat fracture healing callus tissue of vascular endothelial growth factor（ VEGF） expression effect was Studied. 30 healthy SD rats were selected,age 5 - 6 weeks after the numbers were randomly divided into model group,pain swelling group with 15 rats in each group,two groups of SD rats（ Dan Ce）of femur closed fracture model,acetanilide detumescence agent group was treated with Xiaozhongzhitong agent treatment（ 12 h / times）,model group received treatment. The two groups of rats femur as the blank group,the content of VEGF changes of callus were compared between each group number,neovascularization in callus. 2 d,4 d,swelling and pain of live group and the model group rats callus tissues,Were no neovascularization. 7 d,are the formation of new blood vessels in rats of two groups of bony callus,in the number of 10 d were lower in 7 d significantly increased in the 21 d group,the number of new vessels were found in two the decrease in 7、10 and 21 d,the number of new vessels swelling analgesic agent group rats callus tissues were significantly higher than that in the model group rats（ P 〈0. 05）. The 2 d,4 d,21 d number of VEGF live pain swelling agent group and the model group rats bone tissue sections in positive cells don＇t have significant difference（ P 〉0. 05）,7 d,10 d the number of VEGF positive staining cell swelling analgesic agent group rats callus tissues were significantly higher in the model groups of rats（ P 〈0. 05）. Xiaozhongzhitong mixture is conducive to promoting the process of callus formation of blood vessels in rats fracture repair,VEGF expression,which may have some relationship with the promotion of fracture healing in rats.

关 键 词：消肿止痛合剂 大鼠 骨折 骨痂组织 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]