水杨基荧光酮的荧光光谱与荧光量子产率  

Fluorescence Spectra and the Quantum Yield of Salicylfluorone

作　　者：张锋[1] 张洪锋[2] 杨明非[1] 顾皞[1] 

机构地区：[1]东北林业大学理学院,哈尔滨150040 [2]天津科技大学理学院,天津300457

出　　处：《天津科技大学学报》2015年第5期32-36,共5页Journal of Tianjin University of Science & Technology

基　　金：中央高校基本科研业务费专项资金资助项目(2572014BB18)

摘　　要：研究了水杨基荧光酮（SAF）的荧光光谱和荧光量子产率,发现在pH2.0以下,SAF无荧光,随pH升高,SAF由非荧光体转化为荧光体,荧光强度增强,在pH6.0~7.0,SAF有稳定的强荧光,最大发射波长537,nm,最大激发波长507,nm.在碱性条件下,随pH升高,SAF荧光强度下降,当pH在10.0以上SAF无荧光.在pH=6.5的缓冲溶液中,SAF稀水溶液的荧光强度与浓度之间存在良好的线性关系,线性范围为2.0×10^-7~1.2×10^-6,mol/L,检出限为5.2×10^-9,mol/L.SAF在醇溶液中,激发波长蓝移,发射峰红移,Stokes位移增大.在稀水溶液中,β-环糊精对SAF的荧光强度有很强的增敏作用.以罗丹明6,G为参比,使用Williams梯度法测量了SAF的荧光量子产率,在激发波长500,nm处的荧光量子产率为0.68.Fluorescence spectra and fluorescence quantum yield of Salicylfluorone （SAF）have been studied. Under the con- dition of pH〈2.0, SAF has no fluorescence. With the increase of pH value, SAF changes from non-luminous chemical to fluorophore and its fluorescence intensity increases. In the range of pH 6.0-7.0, SAF gives a strong and steady fluorescence with a maximum emission wavelength of 537 nm and excitation wavelength of 507 nm. In alkaline solution, fluorescence intensity of SAF decreases with the increase of pH value, and it has no fluorescence when pH〉 10.0. There is an excellent linear relationship between fluorescence intensity and the concentration of SAF in pH = 6.5 buffer solution. The linear range is 2.0 ×10^-7-1.2 ×10^- 6 mol/L, and the detection limit is 5.2 ×10^-9 mol/L. In alcoholic solutions, Stokes drift of SAF in- creases as blue shift occurs on molecular excitation wavelength and red shift occurs on emission wavelength, fl-cyclodextrin can be used as an excellent sensitizer because fluorescence intensity of SAF is enhanced enormously by adding fl-CD. Rho- damine 6 G was used as a reference, and fluorescence quantum yield of SAF was measured by means of Williams gradient method. At the excitation wavelength 500 nm, the fluorescence quantum yield of SAF is 0.68.

关 键 词：水杨基荧光酮 荧光光谱 荧光量子产率 罗丹明6 G 

分 类 号：O657.39[理学—分析化学]