Cd^(2+)对毛蚶的毒性效应及对其SOD活性和TAOC的影响  

Toxic Effects of Cadmium on Anadara Subcrenata as well as its SOD Activity and Total Antioxidative Competence

作　　者：阎波[1] 李英[1] 高楠[1] 詹志华[1] 高杨[1] 张静雯[1] 

机构地区：[1]天津市海洋资源与化学重点实验室天津科技大学海洋科学与工程学院,天津300457

出　　处：《天津科技大学学报》2015年第5期48-52,共5页Journal of Tianjin University of Science & Technology

基　　金：天津市应用基础与前沿技术研究计划资助项目(15JCYBJC23200);天津科技大学大学生实验室创新基金资助项目(1305A207)

摘　　要：在实验室条件下研究了Cd Cl2对毛蚶的急性毒性效应及对其超氧化物歧化酶(SOD)活性和总抗氧化能力(TAOC)的影响.Cd Cl2对毛蚶24、48、72、96,h的半致死浓度(LC50)分别为24.88、14.52、10.54、6.20,mg/L,随着暴露时间的增加LC50逐渐变小,说明Cd2+对毛蚶的毒性逐渐增加,毛蚶长时间暴露在较低质量浓度的Cd Cl2溶液或短时间暴露在高质量浓度的Cd Cl2溶液中均可产生致死效应.同时测定了不同质量浓度Cd2+(100、500,μg/L)胁迫9,d和清洁海水恢复后毛蚶体内SOD活性和TAOC值的变化,毛蚶体内SOD活性和TAOC值总体被诱导,SOD活性在第9天达到最大值,TAOC诱导率在染毒第7天达到峰值,随后下降.消除Cd2+的胁迫进行清水恢复后,毛蚶SOD活性降低,TAOC变化不明显(P>0.05),表明Cd2+对毛蚶的氧化胁迫作用不会在短时间之内得到恢复.The acute toxicity of CdCl2 on Anadara subcrenata as well as its SOD activity and total antioxidative compe- tence have been studied under experimental conditions. The result showed that the LC50 of cadmium exposed to Anadara subcrenata for 24,48,72 and 96 hours were 24.88, 14.52, 10.54 and 6.20 mg/L, respectively. With the increase of exposure time, the LC50 value decreased gradually, which indicated that the acute toxicity of CdCl2 on Anadara subcrenata increased with the increase of exposure time, and Anadara subcrenata would face lethal risk after being exposed to low concentration CdCI2 solution for a long time or to high concentration CdCl2 solution for a short time. The SOD activity and TAOC value were measured after Anadara subcrenata being exposured to different concentrations （100 μg/L, 500 μg/L） of cadmium sepa- rately for 9 days and then recovered in clean water. The results showed that the SOD activity and TAOC were induced. The SOD activity reached the maximum at the 9th day, the induction rates of TAOC reached the peak value after 7 days con- tamination and then declined. The SOD activity fell after Anadara subcrenata was transferred to clean water, but the differ- ence of TAOC was not significant, which indicated that Anadara subcrenata cannot recover from the stress effects of Cd^2＋ in a short time.

关 键 词：CD^2+ 毛蚶 急性毒性 超氧化物歧化酶(SOD) 总抗氧化能力(TAOC) 

分 类 号：X503.225[环境科学与工程—环境工程]