烟雾暴露对大鼠肺动脉压力与支气管及肺血管PPAR－γ表达的影响  

Effects of cigarette smoke exposure on pulmonary artery pressure and expression of PPAR-γ in bronchus and pulmonary vascular in rats

作　　者：秦艳艳[1] 杜永成[1] 胡晓芸[2] 

机构地区：[1]山西省人民医院呼吸科,太原030012 [2]山西医科大学第一医院呼吸科,太原030001

出　　处：《国际呼吸杂志》2015年第20期1521-1525,共5页International Journal of Respiration

摘　　要：目的探讨不同烟雾暴露量对大鼠肺动脉压力与支气管及肺血管过氧化物酶体增殖物激活受体γ（PPAR-7）表达的影响。方法雄性SD大鼠30只，随机分为对照组、烟雾暴露6周组、烟雾暴露12周组，建立大鼠烟雾暴露模型。检测各组大鼠平均右心室收缩压（mRVSP）；免疫组织化学染色检测支气管上皮细胞及肺血管内皮细胞PPAR-γ的表达量，并分析各指标的相关性。结果①烟雾暴露6周组mRVSP（31．33±14．61）与烟雾暴露12周组（51．00±14．81）均高于对照组（13．43±3．2），差异有统计学意义（F=15．47，P值均〈0．05）；烟雾暴露12周组mRVSP高于烟雾暴露6周组，差异有统计学意义（P〈0．05）；②烟雾暴露6周组与烟雾暴露12周组支气管上皮细胞及肺血管内皮细胞PPAR-7蛋白表达[（0．41±0．01），（O．48±0．08）]与[（0．35±0．01），（0．37±0．07）]均低于对照组[（0．52±0．12），（0．51±0．07）]，差异有统计学意义（F=73．47、58．04，P值均〈0．05）；烟雾暴露12周组支气管上皮细胞及肺血管内皮细胞PPAR-7蛋白表达低于烟雾暴露6周组，差异有统计学意义（P值均〈0．05）；③支气管及肺血管PPAR-γ与mRVSP均呈负相关（r值分别为一0．725和一0．664，P值均〈0．05）。结论烟雾暴露可能通过下调大鼠支气管及肺血管PPAR-γ蛋白的表达，参与肺动脉高压的形成。Objective To investigate the effects of cigarette smoke exposure on pulmonary artery pressure and expression variation of peroxisome proliferator-activated receptor 7 （PPAR-γ） in pulmonary vaseulature and bronchus in rats. Methods Thirty male SD rats were randomly divided into a control group,a smoke exposure for 6 weeks group, a smoke exposure for 12 weeks group, with 10 rats in each group. Mean right ventricular systolic pressure （mRVSP） of each group were measured. The expression of PPAR-γ protein in pulmonary vascular and bronchus of each group were detected by immunohistochemistry. Results ① mRVSP increased significantly in the smoke exposure for 6 weeks group （31.33±14.61） and smoke exposure for 12 weeks group （51.00=[=14.81） compared to the RVSP of control group （13.43±3.2） （ F =15.47, P d0.05, respectively）. The mRVSP in the smoke exposure for 12 weeks group increased significantly compared to the mRVSP of smoke exposure for 6 weeks group （ P 〈 0.05）. ② The expression of PPAR-7 protein in bronchus epithelial cell and pulmonary vascular endothelial cell decreased significantly in the smoke exposure for 6 weeks group[（0.41 ±0.01）, （0.48 ± 0. 08）] and the smoke exposure for 12 weeks group [（0.35±0. 01）,（0. 37±0. 07）] compared to control group [ （0.52 ± 0.12 ）, （0.51 ±0.07） ] （ P 〈 0.05, respectively）. The expression of PPAR-γ protein in bronchus epithelial cell and pulmonary vascular endothelial cell both decreased in the smoke exposure for 12 weeks group compared to the smoke exposure for 6 weeks group （ F = 73.47,58.04, P 〈0.05, respectively）. ③The expression of PPAR-γ protein in bronchus and pulmonary vascular were respectively negatively correlated with mRVSP （ r = --0. 725 and -- 0. 664, P 〈 0.05, respectively）. Conclusions By down- regulating the expression of PPAR-γ protein in bronchus and pulmonary vascular, cigarette smoke exposure induces the increasing of pulmonary artery pressure in rats.

关 键 词：烟雾暴露 肺动脉压 过氧化物酶体增殖物激活受体Γ 

分 类 号：R563[医药卫生—呼吸系统]