固氮解磷菌RSN219和解磷苜蓿根瘤菌LW107对红豆草及燕麦幼苗生长的影响  

Effects of RSN219(Klebsiella pneumoniae)and LW107(Rhizobium meliloti)on Growth of Onobrychis viciaefolia and Avena sativa Seedlings

作　　者：李剑峰[1] 张淑卿[1] 刘攀道[2] 杜建雄[3] 韩宝银[1] 

机构地区：[1]贵州师范学院贵州省生物资源开发利用特色重点实验室/喀斯特生境土壤与环境生物修复研究所,贵州贵阳550018 [2]中国热带农业科学院品种与资源研究所,海南儋州571737 [3]贵州财经大学环境资源学院,贵州贵阳550025

出　　处：《贵州农业科学》2015年第9期105-111,共7页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"石漠先锋植物金银花-根际解磷菌的协同解磷机理研究"(31300389);贵州省教育厅重点实验室建设项目"生物资源开发利用特色重点实验室"(2012156);贵州省科技基金项目"基于植物源抑菌剂的解磷根瘤菌抗污染菌剂制备研究"[J(2014)2137]

摘　　要：为了解固氮解磷菌和根瘤菌对红豆草和燕麦的促生效应,用砂培盆栽法研究固氮解磷菌RSN219(Klebsiellasp.)和苜蓿解磷根瘤菌LW107(Rhizobium meliloti)对红豆草和燕麦幼苗生长的影响。结果表明:在无氮,仅提供低有效磷(仅提供磷酸钙)的栽培条件下,接种RSN219和LW107菌株不但能够缓解甘肃红豆草和燕麦Calibre由于氮、磷缺乏导致的生长受抑,而且对2种牧草的幼苗生长有明显的促进作用,使2种牧草的株高、根长、单叶面积、单株叶片数、根体积、植株体积、地上鲜干重、地下鲜干重、植株鲜干重、根冠比(R/T)和干鲜重比(D/F)高于或显著高于(P<0.05)不接种菌液亦不施入完全营养液的CK1和仅施入1/4完全营养液的CK2。可见,解磷苜蓿根瘤菌R.meliloti LW107和解磷固氮菌K.pneumoniae RSN219对非宿主植物均有良好的促生效果,具有良好的应用前景,其中R.meliloti LW107促生作用弱于RSN219。The effect of nitrogen-fixing and phosphate-solubilizing bacteria RSN219（Klebsiella pneumoniae）and rhizobium LW107（Rhizobium meliloti）on growth of Onobrychis viciaefolia and Avena sativaseedlings to discuss the promotion effect of nitrogen-fixing and phosphate-solubilizing bacteria and rhizobium on O.viciaefoliaand A.sativaseedlings.Results：Under the cultivation conditions of nitrogenfree and low available P level,inoculating RSN219 and LW107strains not only relieves suppression of O.viciaefoliaand A.sativa seedlings＇ growth because of insufficient N and P,but also has the obvious promotion effect on growth of O.viciaefolia and A.sativa seedlings.Height,root length,single-leaf area,leaves per plant,root volume,plant volume,fresh and weight of overground part,fresh and dry weight of underground part,fresh and dry weight of seedling,root top ratio and dry/fresh weight ratio of O.viciaefoliaand A.sativaseedlings inoculated with RSN219 and LW107strains are significantly higher than CK1（without inoculating RSN219 and LW107strain＋ without applying complete nutrition solution）and CK2（without inoculating RSN219 and LW107strain ＋ applying 1/4complete nutrition solution）respectively.In conclusion,R.meliloti LW107 and K.pneumoniae RSN219 have the good promotion effect on non-host plants and the good application prospect.The promotion effect of R.meliloti LW107 is lower than K.pneumoniae RSN219.

关 键 词：固氮解磷菌 根瘤菌 红豆草 燕麦 幼苗生长 

分 类 号：S431.14[农业科学—农业昆虫与害虫防治]