半夏块茎腐烂病原细菌拮抗菌的分离与鉴定  

Isolation and Identification of Antagonistic Bacteria against Pectobacterium carotovorumin Pinelia ternate

作　　者：张洁[1] 胡琨敏 李玉权[1] 任建国[1] 任春丽[1] 刘红美[1] 

机构地区：[1]贵州医科大学生物技术教研室,贵州贵阳550025

出　　处：《贵州农业科学》2015年第9期150-152,156,共4页Guizhou Agricultural Sciences

基　　金：贵州省中药现代化科技产业研究开发专项"贵州半夏块茎腐烂病防治体系关键技术研究与应用示范"[黔科合成字(2013)5014];国家级大学生创新创业训练计划项目"软腐病拮抗菌的筛选和鉴定"(201410660007);贵州省科技厅联合基金项目"防治半夏块茎腐烂病微生物有机肥研制与效应"[黔科合LH字(2014)7094]

摘　　要：为促进贵州半夏发展绿色种植,采用平板稀释法和平板对峙培养法从贵州省不同地区采集的土壤样品中筛选对半夏块茎腐烂病病原菌具有较强拮抗效果的生防菌株,结合形态学观察、生理生化检测和16SrRNA序列分析对其进行鉴定。结果表明:采集土样中共分离得到270株细菌,从中筛选到1株有较强拮抗活性的细菌HM9-1,其对块茎腐烂病病原菌胡萝卜软腐果胶杆菌胡萝卜软腐亚种的抑菌圈直径达2.4cm。经鉴定,该菌株为巨大芽孢杆菌。The biocontrol strains with more strong antagonistic effects on P.carotovorumof P.ternate were screened from soil samples of various regions in Guizhou by plate dilution and plate confront culture methods and then the isolated strains were identified by morphological observation,physiological and biochemical detection and 16 SrPNA sequence analysis to promote green cultivation of P.ternate in Guizhou.Results：270bacteria strains are isolated and a strain,HM9-1 with more strong antagonistic effect,is screened from 270 bacteria stains.HM9-1strain with 2.4cm of inhibition zone against P.carotovorumis identified as B.megatherium.

关 键 词：半夏 胡萝卜软腐果胶杆菌胡萝卜软腐亚种 拮抗细菌 巨大芽孢杆菌 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]