石墨烯基固相萃取灵敏检测环境水样中痕量罗丹明B  

Sensitive detection of trace rhodamine B in environmental water based on graphene solid phase extraction

作　　者：李惠茗[1] 李琴[1] 赖祥文[1] 梁立[1,2] 刘坤平[1,2] 苟小军[2] 

机构地区：[1]成都学院生物产业学院,成都610106 [2]成都学院,药食同源植物资源开发四川省高校重点实验室,成都610106

出　　处：《分析试验室》2015年第10期1151-1154,共4页Chinese Journal of Analysis Laboratory

基　　金：四川省中医药管理局中医药科学技术研究专项(2014F002);四川省教育厅科研项目(15ZB0374);成都大学大学生创新性实验计划项目(CDU_CX_201409)资助

摘　　要：采用石墨烯作为吸附剂制备固相萃取（SPE）小柱,结合高效液相色谱用于环境水样中痕量罗丹明B的灵敏检测。由于大比表面积的石墨烯与罗丹明B之间具有强烈的π-π共轭作用,可提高罗丹明B在石墨烯上的吸附量,从而增强所制备的石墨烯基SPE小柱对罗丹明B的富集能力。对固相萃取条件进行优化,在优化的条件下采用Inertsil ODS-SP（250 mm×4.6 mm,5μm）色谱柱,水：甲醇=25：75（V/V）做为流动相,流速1.0 m L/min,进样量20μL,550 nm波长下进行检测。该方法在3.91~250.0μg/L范围内线性关系良好,检出限（3σ）为2.29μg/L。该SPE小柱对罗丹明B具有良好的选择性富集能力,可以实现环境水样中痕量罗丹明B的准确和灵敏检测。In this paper,graphene was used as solid-phase extraction（ SPE） adsorbent for the extraction of trace Rhodamine B（ RB） in environmental water body. Combined with the high-performance liquid chromatography（ HPLC）,a sensitive detection of RB was achieved. Due to the large special surface area of graphene and intensive π- π conjugation between graphene and RB,the loading amount of RB was increased,and thus the enrichment ability of as-prepared graphene-based SPE column for RB was enhanced. Under the optimal SPE conditions,the Inertsil ODS-SP（ 250 mm × 4. 6 mm,5 μm） chromatographic column was used for sample detection with mobile phase of methanol vs water（ 75： 25,1. 0 m L / min）,injection volume of 20 μL and detection wavelength of 550 nm. This method exhibited a wide linear range of 3. 91 ~ 250. 0 μg / L and low detection limit of 2. 29 μg / L（ 3σ）. Moreover,the proposed method showed good precision and stability,and could be used for the sensitive detection of RB in environmental water body. Therefore,the present strategy definitely paves a way for trace component detection in environmental samples and broadens the application of graphene.

关 键 词：固相萃取(SPE) 高效液相色谱(HPLC) 石墨烯 罗丹明B 

分 类 号：O657.7[理学—分析化学]