一种挽救轻度污染的肿瘤贴壁细胞的方法  

A method of saving the slightly contaminated adherent tumor cells

作　　者：姬明丽[1] 王煜霞[2] 

机构地区：[1]新乡医学院生理学教研室,河南新乡453003 [2]新乡医学院病理生理学教研室,河南新乡453003

出　　处：《新乡医学院学报》2015年第9期814-816,共3页Journal of Xinxiang Medical University

基　　金：河南省基础与前沿技术研究计划项目(编号:132300410160);河南省高校青年骨干教师资助计划项目(编号:2011GGJS-127);新乡医学院重点研究领域招标课题资助项目(编号:ZD2011-19)

摘　　要：目的探讨一种挽救轻度污染的贴壁肿瘤细胞的方法。方法经多次双抗培养液吹打离心、双抗磷酸盐缓冲液冲洗处理的轻度污染的鼻咽癌细胞株XB-5-8F、鼻咽癌细胞株C666-1和食管鳞状细胞癌细胞系TE8等贴壁细胞(实验组)进行传代培养48 h,复苏的相应冻存细胞(对照组)传代培养48 h,对2组细胞计数、细胞活力及细胞形态等进行比较。结果实验组细胞处理后总细胞计数、活细胞计数及细胞活力均大于处理前,死亡细胞计数低于处理前,差异均有统计学意义(P<0.05)。实验组和对照组总细胞计数、活细胞计数、细胞活力及细胞形态等比较差异均无统计学意义(P>0.05)。实验组和对照组3种细胞的吸光度值比较差异无统计学意义(P>0.05)。结论此种对轻度污染细胞的处理方法可以恢复细胞活性,完成细胞传代。Objective To establish a viable method to save the slightly contaminated adherent tumor cells. Methods The XB-5-8 F,C666-1 and TE8 cells growed in Roswell Park Memorial Institute（ RPMI） medium 1640 supplemented with newborn calf serum and two antibiotics,and these cells were beated by pipett,washed by phosphate buffer saline with two antibiotics and centrifugated repetitively; these cells as experimental group. Cryopreserved XB-5-8 F,C666-1 and TE8 cells were resuscitated as control group. All cells of two groups have been subcultured 48 hours. Then cell count,cell viability and cell morphology were compared between the two groups. Results After treatment,the total cell counts,viable cell counts,cell viability were higher than before treatment,and the dead cell counts were lower than before treatment in experiment group（ P〈0. 05）. There were no statistic difference of the total cell counts,viable cell counts,cell viability and cell morphology between the experiment group and control group（ P〉0. 05）. There were no statistic difference of optical density value of the XB-5-8F,C666-1 and TE8 cells between the experiment group and control group（ P〉0. 05）. Conclusion This treatment method can restore cell activity,and these treated cells can be cultured and passaged stably.

关 键 词：肿瘤贴壁细胞 轻度污染 传代 

分 类 号：Q813[生物学—生物工程]