丹参注射液联合曲安奈德对口腔黏膜下纤维性变组织中肌成纤维细胞生物学行为的影响  

Effects of Salvia Miltiorrhiza Injection Combined with Triamcinolone Acetonide on Myofibroblasts in Oral Submucous Fibrosis

作　　者：朱超[1] 孙璐[1] 闵安杰[1] 蒋灿华[1] 

机构地区：[1]中南大学湘雅医院口腔颌面外科,湖南长沙410008

出　　处：《中医药导报》2015年第20期3-6,20,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81102045);湖南省科技厅基础研究项目(No.2011FJ3148)

摘　　要：目的:研究丹参注射液联合曲安奈德对口腔黏膜下纤维性变(OSF)组织中肌成纤维细胞(MFs)生物学行为的影响,为两种药物联合使用治疗OSF探明机理。方法:采用组织块培养法分离培养OSF患者病变组织中的MFs,同法分离培养健康志愿者正常颊黏膜中的成纤维细胞。以不同浓度的丹参注射液联合曲安奈德作用于MFs,采用MTT法检测细胞增殖水平,羟脯氨酸试剂盒检测细胞分泌可溶性胶原含量的变化,免疫细胞化学法检测细胞中α-SMA的表达情况。以槟榔碱提取液刺激正常成纤维细胞,保护组加用丹参注射液联合曲安奈德,同法检测其α-SMA的表达情况。结果:不同浓度的丹参注射液联合曲安奈德可明显抑制MFs的增殖水平及胶原分泌水平,并可使其α-SMA的表达水平明显较低,还可抑制槟榔提取物促进正常成纤维细胞表达α-SMA的作用。结论:丹参注射液联合曲安奈德可抑制OSF组织中MFs的部分生物学行为,可促使其向正常成纤维细胞逆分化,并能阻止正常成纤维细胞转分化为MFs,对口腔黏膜下纤维性变患者具有一定的治疗和保护作用。Objective： To investigate the effects of Salvia Miltiorrhiza Injection（SMI） and Triamcinolone Acetonide on bio-logical behavior of myofibroblasts（MFs） in oral submucous fibrosis（OSF） and explore its mechanism in treatment of OSF.Method： MFs were isolated from OSF patients＇ buccal mucosa and incubated with various concentrations of SMI and triamci-nolone acetonide. The expression of α-SMA in MFs and NFs were detected by immunocytochemical method and RT-PCR. MTT assay and hydroxyproline reagcuat were used to evaluate the cell proliferation and collagen production. Results： SMI combined with Triamcinolone Acetonide could not only significantly inhibit the proliferation of MFs and the expression of α-SMA, but also reduce the collagen production in the culture medium. Conclusion： SMI combined with Triamcinolone Acetonide could reduce the proliferation and collagen production of MFs and inhibit the transdifferentiation of NFs to MFs, and suggested that SMI com-bined with Triamcinolone Acetonide could play protective and therapeutic role in the treatment of OSF.

关 键 词：口腔黏膜下纤维性变(OSF) 肌成纤维细胞(MFs) 丹参注射液 曲安奈德 槟榔碱 

分 类 号：R285.5[医药卫生—中药学]