HPLC法同时测定夏枯草消瘤合剂中迷迭香酸和咖啡酸的含量  

Simultaneous Determination of Rosmarinic Acid and Caffeic Acid in Xiakucao Xiaoliu Mixture by HPLC

作　　者：朱全刚[1] 程亮[1] 顾小燕[1] 张若曦[1] 张玉萱[1] 韩克起[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《中医药导报》2015年第20期21-23,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市科委中医重点项目(No.13401902002);上海市卫计委进一步加快中医药事业发展三年行动计划(2014年-2016年)项目(No.ZY3-JSFC-2-3006)

摘　　要：目的：建立同时测定夏枯草消瘤合剂中迷迭香酸、咖啡酸含量的方法。方法：采用HPLC法,色谱柱为Agilent1200-C18（4.6 mm×250 mm,5μm）,流动相为甲醇（A）-1.0%甲酸水溶液（B）,梯度洗脱（0~11.0 min,30%A;11.0~11.1min,30%A→45%A;11.1~23.0 min,45%A;23.0~23.1 min,45%A→30%A;23.1~30.0 min,30%A）,流速为1.0 m L/min,检测波长为330 nm,柱温为25℃,进样量为20μL。结果：迷迭香酸和咖啡酸的质量浓度分别在1.06~74.2μg/m L、1.05~73.5μg/m L范围内与各自峰面积呈良好的线性关系（r均为0.9999）;平均加样回收率分别为98.86%、98.33%,RSD%分别为2.95%、2.56%。6批样品中迷迭香酸、咖啡酸的平均含量分别不低于0.185 mg/m L和0.027 mg/m L。结论：该方法简便、快速、准确,重复性好,可用于夏枯草消瘤合剂的质量控制。Objective： To establish a method for simultaneous determination of rosmarinic acid and caffeic acid in Xiakucao Xiaoliu Mixture. Method： HPLC method was adopted. The determination was performed on Agilent1200-C18（4.6 mm×250 mm,5 μm） column with mobile phase consisted of methanol（A）-1.0% formic acid solution（B） at the flow rate of 1.0 m L/min（gra-dient elution, 0~11.0 min, 30% A; 11.0~11.1 min, 30% A→45% A; 11.1~23.0 min, 45% A; 23.0~23.1 min,45% A→30% A;23.1~30.0 min,30% A）. The detection wavelength was set at 330 nm and column temperature was 25 ℃. Injection Volume was 20μL. Results： The linear ranges were 1.06~74.2 μg/m L for rosmarinic acid and 1.05~73.5 μg/m L for caffeic acid（both r=0.9999）.Average recoveries of them were 98.86%（RSD=2.95%, n=6） and 98.33%（RSD=2.56%, n=6）. The average contents of them were not less than 0.185 mg/m L and 0.027 mg/m L in 6 batches of samples. Conclusion： The method is proved to be simple, rapid,accurate, and reproducible, and can be used for the quality control of Xiakucao Xiaoliu Mixture.

关 键 词：夏枯草消瘤合剂 迷迭香酸 咖啡酸 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]