微波辅助提取秦岭龙胆挥发油的工艺优化及GC-MS分析  

Microwave-assisted Extraction Method and GC-MS Analysis of Volatile Oil from Gentiana Apiata N. E. Br.

作　　者：许海燕[1] 郑伶俐 

机构地区：[1]陕西国际商贸学院医药学院,陕西咸阳712046 [2]步长集团咸阳制药厂,陕西咸阳7120003

出　　处：《中医药导报》2015年第20期31-35,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省教育厅项目(2013JK0804)

摘　　要：目的:优选微波辅助提取秦岭龙胆挥发油的工艺,并对挥发油成分进行GC-MS分析。方法:以挥发油提取率为指标,通过单因素试验结合响应面法考察微波功率、提取时间、液料比对秦岭龙胆挥发油的微波辅助萃取工艺的影响,并通过GCMS对秦岭龙胆挥发油中化学成分进行分析。结果:各因素对挥发油提取率的影响顺序为:微波功率>液料比>提取时间;最佳工艺参数为:微波功率432 W,提取时间10 min,料液比12:1(m L/g),在该条件下挥发油得率为3.383%。GC-MS分析鉴定出29种挥发油,其中主要有油酸(10.29%)、亚油酸(9.04%)、棕榈酸(6.23%)、硬脂酸(3.48%)等。结论:微波辅助提取法用于提取秦岭龙胆挥发油具有时间短、收率高、节约能耗等优点,优化的工艺稳定可靠,通过GC-MS分析其挥发油也是秦岭龙胆中的活性成分之一,这将为秦岭龙胆的进一步综合开发利用提供科学依据。Objective： To optimize microwave-assisted extraction process of volatile oil from Gentiana apiata N. E. Br.,and to analyze the components of volatile oil by GC-MS. Method： With yield of volatile oil as index,effects of microwave power、extraction time and solid-to-liquid ratio process of volatile oil from Gentiana apiata N. E. Br. were investigated by single factor test and response surface method, and chemical constituents of volatile oil were analyzed by GC-MS. Results： Effects of three factors on yield of volatile oil were in order of microwave power solid-to-liquid ratio extraction time; Optimal fluid extraction technology was as following： microwave power 432 W,liquid-to-solid 12：1（m L/g）,extraction time 10 min;Yield of volatile oil under these conditions was 3.383%. 29 compounds were identified by GC-MS; the main compounds were Oleic acid（10.29%）,linoleic acid（9.04%）, palmitic acid（6.23%）, stearic acid（3.48%） and so on. Conclusion：Microwave-assisted method had higher extraction efficiency, can save time and has low energy consumption. This optimized technology was stable and reliable. In addi-tion, chemical compositions of volatile oil were complicated and volatile oil, one of the major bioactive components from Gen-tiana apiata N. E. Br. by GC-MS. The results provide the fundamental data for the further development of Gentiana apiata N.E. Br.

关 键 词：秦岭龙胆 微波辅助提取 挥发油 响应面法 成分分析 

分 类 号：R284.1[医药卫生—中药学]