金纳米棒的偏光特性及其作为方向探针在单分子水平成像上的应用  

Polarization properties of gold nanorod and its applications in singlemolecule level imaging as a direction probe

作　　者：杨玉东[1] 刘公召[1] 李冬至[1] 徐菁华[1] 杨林梅[1] 

机构地区：[1]沈阳工业大学理学院,沈阳110870

出　　处：《中国科学：化学》2015年第10期1010-1025,共16页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(50375102);沈阳工业大学博士后启动基金(521101302)资助

摘　　要：随着生物医学的发展,对生物成像技术和成像分辨率的要求越来越高,纳米材料和技术被越来越多地应用到生物医学领域.各向异性的金纳米棒由于具有较高的电子密度、较大的吸收截面、特殊的表面等离子共振光学特性、优良的生物相容性和化学稳定性而被广泛应用于生物成像领域.复杂的活细胞组织内纳米级目标的位置和动态的空间构型对于我们理解很多生物物理学过程的细节问题至关重要.本文结合本课题组在该领域的研究经验,并从金纳米棒局部等离子共振特性和偏光特性出发,综述了金纳米棒作为方向探针的3D跟踪定位成像技术与手段.包括暗场成像技术、微分干涉成像、光热成像、共聚焦显微镜成像等生物成像技术和脱焦成像、全内反成像和双通道等生物成像手段.同时阐述了金纳米棒作为方向探针在生物成像领域中的应用进展.With the development of biomedicine, since higher resolution capability of biomedical imaging system is needed, the nanometer material and technology are increasingly applied in biomedical fields. Due to the high electron density, large absorption cross section, special surface plasma resonance, good bio-compatibility and chemical stability, anisotropic gold nanorods are widely used in the field of biological imaging. It is essential for us to understand the details of a lot of biophysics process with the position and dynamic space imaging of the nanoscale target inside the complex living cells. In this paper, from the local plasma resonance of gold nanorods and polarization characteristics, we summary the tracking 3D imaging techniques and methods of gold nanorods as a direction probe, with our experience in the field of study. These details include the techniques of dark field scattering imaging, differential interference, photo thermography and confocal microscopy, and the methods of bokeh imaging, total internal reflection imaging and dual channels. Finally, the application progress of gold nanorods as a direction probe in biological imaging is described.

关 键 词：金纳米棒 生物医学 单分子方向 三维示踪 

分 类 号：R318[医药卫生—生物医学工程] O614.123[医药卫生—基础医学]