吉林省2013年柯萨奇病毒A组16型基因特征分析  

Analysis of CVA16 gene characteristics in Jilin in 2013

作　　者：魏雷雷[1] 周剑惠[2] 王爽[1] 李可维[2] 单元春[2] 范明[2] 

机构地区：[1]吉林省卫生监测检验中心,吉林长春130062 [2]吉林省疾病预防控制中心,吉林长春130062

出　　处：《中国卫生检验杂志》2015年第19期3309-3311,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家科学技术艾滋病和病毒性肝炎等重大传染病防治专项传染病监测技术平台(2012ZX10004209-003)

摘　　要：目的了解吉林省2013年柯萨奇病毒A组16型（CVA16）流行特征、种系进化及基因分型。方法对吉林省2013年分离获得的9株CVA16阳性毒株的VP1编码区核苷酸进行扩增及序列测定,使用Mega 4.0和Bioedit软件进行序列分析。结果 9株CVA16流行株中,1株为B1a基因亚型,与3株B1a代表株相比,其核苷酸和氨基酸的同源性分别为95.6%~96.8%和99.6%;8株为B1b基因亚型,与3株B1b代表株相比较,其核苷酸和氨基酸的同源性分别为94.1%~96.1%和98.3%~100%。2013年流行株与代表株相比共发生了9处氨基酸位点变异,其中8株B1b亚型中有6株在第23位氨基酸位点发生了亮氨酸→蛋氨酸的变异。结论 B1b是引起吉林省2013年CVA16流行的主要基因亚型,并且有大部分CVA16流行株在第23氨基酸位点发生了共同变异。Objective To understand the coxsackievirus group A type 16（ CVA16） epidemic characteristics,phyletic evolution and genotyping in Jilin in 2013. Methods To amplify and analyze the sequence of the nucleotide in VP1 of 9 CVA16 positive strains isolated from Jilin in 2013,and used Mega 4. 0 and Bioedit software for sequence analysis. Results Out of the 9CVA16 strains,1 strain was B1 b subtype,and compared with the other 3 B1 b representative strains,its homology of nucleotide and amino acid were 95. 6%- 96. 8% and 99. 6%; 8 strains were B1 b subtypes,and compared with the other 3 B1 b representative strains,their homology of nucleotide and amino acid were respectively 94. 1%- 96. 1% and 98. 3%- 100%. Of the epidemic strains,compared with the representative strain,mutation occured on 9 amino acid sites,and out of 8 B1 b subtypes,the variation of Leucine→Methionine happened on the 23 amino acid site in 6 strains. Conclusion B1 b is the major gene subtype of CVA16 in Jilin in 2013,and there is a common variation on the 23 amino acid site of most CVA16 epidemic strains.

关 键 词：柯萨奇病毒A组16型 手足口病 VP1编码区 氨基酸变异 

分 类 号：R373.23[医药卫生—病原生物学]