吉林省2012～2013年<5岁婴幼儿中检出诺如病毒GⅡ.4Sydney(悉尼)2012变异株  

Detection of Norovirus Genotype GⅡ. 4 Sydney2012 Variant from Children under 5 Years of Age in Jilin Province,2012-2013

作　　者：李响[1] 杨显达[1] 许爽[1] 吴东林[1] 孙利炜[2] 沈博[1] 李静[1] 柳鸿敏[1] 

机构地区：[1]吉林省疾病预防控制中心,长春130062 [2]长春市儿童医院,吉林长春130061

出　　处：《中国疫苗和免疫》2015年第3期283-288,共6页Chinese Journal of Vaccines and Immunization

基　　金：国家科学技术艾滋病和病毒性肝炎等重大传染病防治重大专项传染病监测技术平台项目辽宁及周边省市传染病病原谱流行规律研究课题吉林省传染病病原谱流行规律研究子课题(编号:2012ZX10004209-003);吉林省卫生和计划生育委员会科学研究项目(课题编号:2014ZC039)

摘　　要：目的了解吉林省2012~2013年诺如病毒（Norovirus,NV）流行型别及感染状况。方法收集长春市儿童医院2012~2013年〈5岁住院腹泻患儿的粪便标本,采用逆转录-聚合酶链反应（Reverse Transcription-Polymerase Chain Reaction,RT-PCR）检测粪便标本中的NV,阳性标本进行核苷酸序列测定,构建系统进化树进行分析。结果检测粪便标本共770份,NV阳性139份,总阳性率为18.1%,其中2012年总阳性率为18.6%（72/387）,2013年总阳性率为17.5%（67/383）,〈2岁婴幼儿感染占总标本数的97.1%,全部为基因Ⅱ组（GenogroupⅡ,GⅡ）。PCR产物进行序列测定,取得测序结果的共97份,其中GⅡ.4型53份,占54.6%;GⅡ.3型41份,占42.3%;GⅡ.13型2份,占2.1%;GⅡ.6型1份,占1.0%。2012年检出GⅡ.4 Sydney（悉尼）2012变异株2份,占2012年阳性总数的4.9%;2013年检出26份,占2013年阳性总数的46.4%。结论吉林省存在多种NV基因型的感染,2013年流行优势株为GⅡ.4 Sydney2012变异株。Objective To describe the genotypes and situation of norovirus （NV） infection in Jilin prov- ince in 2012 and 2013. Methods Stool samples from children under 5 years of age with diarrhea were collected from Changchun Children＇s Hospital. We detected NV using reverse transcription-polymerase chain reactions （RT-PCR）. Positive samples were sequenced and the phylogenetic tree was constructed for analysis. Results In total, 139 of 357 stool samples were positive for NV ribonucleic acid. The total positive rate was 18.6% in 2012 and 17.5% in 2013. Children under 2 years of age accounted for 97. 1% of the all infected samples, and all strains belonged to genogroup II （G Ⅱ ）. The PCR products were sequenced, and for 97 samples we obtained longed to genotype GⅡ. 4; 41 strains （42.3%） longed to genotype GⅡ. 13 ; and 1 strain （ 1.0% ） 2012 strains were detected in 2012, accounting for ney2012 strains were detected in 2012, accounting sequencing results. Fifty-three strains （54.6%） be- belonged to genotype G Ⅱ. 3; 2 strains （2. 1% ） be- belonged to genotype GⅡ. 6. Two mutant GⅡ. 4 Sydney 4. 9% of positive samples. Twenty-six of the GⅡ. 4 Syd- for 46. 4% of the total positive samples. Conclusion There were many kinds of NV genotype infections in Jilin province, and G II. 4 Sydney2012 strain became the predominant one in 2013.

关 键 词：〈5岁婴幼儿 诺如病毒 基因型 GⅡ.4 Sydney(悉尼)2012变异株 

分 类 号：R373.11[医药卫生—病原生物学] R183.3[医药卫生—基础医学]