局部低温联合生脉与地塞米松对睾丸扭转复位后生精细胞凋亡的保护作用  

Protective effects of hypothermia union Shengmai and dexamethasone on the spermatogenic cell apoptosis of torsioned testes in rats

作　　者：张家彬[1] 陈光炳[1] 翁吴斌 张春[1] 

机构地区：[1]福建医科大学附属闽东医院泌尿外科、宁德市泌尿外科研究所,福建福安355000

出　　处：《中国临床药理学杂志》2015年第20期2037-2039,共3页The Chinese Journal of Clinical Pharmacology

基　　金：福建省宁德市科技计划指导基金资助项目(20110094)

摘　　要：目的研究生脉、地塞米松联合局部低温对大鼠睾丸扭转复位后生精细胞凋亡的影响。方法将48只青春期标准SD大鼠随机分为6组(A、B、C、D、E、F组),每组8只。6组大鼠分别扭转左侧睾丸720°持续2 h,建立单侧睾丸扭转模型。A组:常温+生理盐水;B组:常温+生脉+地塞米松;C组:低温+生理盐水;D组:低温+地塞米松;E组:低温+生脉;F组:低温+生脉+地塞米松。术后48 h采集睾丸,通过HE染色光镜观察睾丸组织病理学改变,用原位缺口末端标记法(TUNEL)法检测睾丸生精细胞的凋亡。结果 B、C、D、E组,睾丸组织可见部分生精细胞脱落;F组,小鼠睾丸内出现生精细胞脱落现象。凋亡指数(AI),A组(35.13±2.21)明显高于B组(18.12±5.23)、C组(17.13±3.03)、D组(17.05±1.03)、E组(16.39±2.02;均P<0.05)及F组(9.06±1.53;P<0.01)。结论睾丸扭转复位后缺血再灌注损伤可导致生精细胞凋亡增加;局部低温联合生脉、地塞米松对睾丸扭转复位后生精细胞凋亡具有良好保护作用。Objective To investigate the protective effect of hypothermia union Shengmai and dexamethasone on spermatogenie cell apoptosis of torsioned testes in rats. Methods Forty- eight adolescence male Spra- gue -Dawley rats were random divided into 6 groups. Each group of 8 rats, six group rats were left testieular torsion 720° for 2 h, establish a u- nilateral testicular torsion model. Group A ： normal temperature ＋ saline ; group B ： normal ＋ Shengmai ＋ dexamethasone ; group C ~ low temperature ＋ saline ; group D ： low temperature ＋ dexamethasone ; group E ： low tem- perature ＋ Shengmai ; group F： Shengmai ＋ dexamethasone ＋ low tempera- ture. The testicles were collected after 48 h, the testicular histopathologi- cal changes were observed by HE staining light microscopy. The apopto- tic spermatogenic cells law were detected by TdT -mediated dUTP nick -end labeling （TUNEL） . Results Group B, C, D and E testicular tissue pathology damage visible parts of pure cell falls off born. The born small tube of testicular sperm cells in group F is born slightly off, general structure and tidy. Apoptosis index （AI） in group A （ 35.13 ± 2. 21 ） was higher than that group B （ 18. 12 ± 5.23 ）, group C （ 17.13 ±3.03 ）, groupD （17.05±1.03）, group E （16.39±2.02;allP〈0.05） and group F （ 9.06 ± 1.53 ; P 〈 0.01 ） . Conclusion Testicular torsion reduction ischemia- reperfusion injury can lead to life essence cell apoptosis increase. Hypothermia union Shengmai and dexa-methasone on spermatogenic cell apoptosis of torsioned testes in rats have good protection.

关 键 词：睾丸扭转 低温 生脉 地塞米松 生精细胞凋亡 

分 类 号：R978.1[医药卫生—药品] R965.1[医药卫生—药学]