RPS27L基因作为辐射损伤标志物的研究  

Study on RPS27L gene as a marker of radiation damage

作　　者：李孝鑫 扈江伟[3] 张学清[2] 沈丽萍[2] 李琳[2] 常诚[2] 王治东[2] 陈英[1,2] 

机构地区：[1]安徽医科大学,合肥230032 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]解放军307医院,北京100071

出　　处：《辐射研究与辐射工艺学报》2015年第5期30-36,共7页Journal of Radiation Research and Radiation Processing

基　　金：全军医学科技"十二五"科研项目(BWS11J018);全军后勤科研重大项目(AWS14C014);解放军307医院院创新基金(JS-2014-04)项目资助

摘　　要：以人外周血为研究对象,研究照射后RPS27L基因的时间效应和剂量效应以及本底水平,探究其作为辐射损伤早期生物标志物的可行性。采集3名健康成人外周血,利用γ射线对其进行照射至吸收剂量分别为0、0.5、1、2、4、6和10 Gy,受照后37℃培养2、4、8、12和24 h,利用实时荧光定量PCR检测RPS27L基因的表达变化,并分析在37个健康人个体中的本底水平。结果显示,受照后的4、8、12和24 h RPS27L基因表达变化显著,而且具有很好的剂量依赖关系;在不同年龄和性别的个体中RPS27L本底水平相对稳定。因此,RPS27L基因具有作为新的辐射损伤标志物的潜力。In order to explore RPS27L＇s possibility as a biomarker of radiation damage, we studied its time-response and dose-response after irradiation as well as its background level. Three healthy adults＇ peripheral blood was exposed to gamma-rays with 0, 0.5, 1, 2, 4, 6, and 10 Gy, separately and cultured for 2, 4, 8, 12, and 24 h respectively. RPS27L＇s expression change was detected by quantitative real-time PCR. We also analyzed its background level in 37 individuals. The results showed that RPS27L had a significant difference at 4, 8, 12, 24 h and a great dose-independent response. There was also a uniform background level of RPS27L among different age and gender. Thus, RPS27L is of great potential to be a new biomarker of radiation.

关 键 词：核辐射 RPS27L 基因表达变化 实时荧光定量PCR 生物标志物 

分 类 号：TL71[核科学技术—辐射防护及环境保护]