血清VGEF、TNF-α的表达与慢性血栓栓塞性肺动脉高压大鼠肺血管重构的相关性研究  

Impact on chronic thromboembolic pulmonary vascular remodeling in rats with pulmonary hypertension inflammation

作　　者：王佐岩[1] 孙平军[1] 张一梅[2] 

机构地区：[1]大庆龙南医院呼吸内科,黑龙江大庆163453 [2]哈尔滨医科大学附属第一医院呼吸内科

出　　处：《中国伤残医学》2015年第21期11-13,共3页Chinese Journal of Trauma and Disability Medicine

摘　　要：目的：研究慢性血栓栓塞性肺动脉高压（ chronic thrombo embolic pulmonary hypertension,CTEPH）炎症因子表达水平的动态变化以评估炎症反应与肺血管重构的关系. 方法：雄性 Wistar大鼠64 只,随机分为8 组,每组8 只：初始对照组（ IC组）、栓塞3 天组、1 周组、2 周组、4 周组、8 周组、12 周组（ D3、W1、W2、W4、W8、W12 ）、终末对照组（ EC组）. 采血制备0. 5 mm左右大小的自体血栓,颈静脉注入27 ～30 个自体血栓,2 周后第2 次注入同样数目血栓,2 次术后肌注庆大霉素预防感染,全程腹腔注射氨甲环酸. 达各组实验目标日期后,测各组大鼠平均肺动脉压（ mPAP）、肺动脉相对中膜厚度（ PAMT）、管壁面积/管总面积（ WA/TA）百分比,免疫组化检测血管内皮生长因子（ VGEF）、肿瘤坏死因子（ TNF-α）表达. 结果：第1 次栓塞后mPAP略有升高,与对照组比较无统计学差异（P〉0. 05）,第2 次栓塞后随时间延长mPAP明显增高（均P〈0. 01）;PAMT、WA/TA在4 周后随时间延长显著增高（4 周组P〈0. 05,8 周、12 周组P〈0. 01）;不同时间组血清VGEF、TNF-α值较初始对照组和终末对照组均明显升高,P〈0. 01,差异有统计学意义,其中 W1 达峰值,其后有所下降,2 次栓塞后W4 再次达峰值,其后维持较高水平. 相关分析表明,VGEF与TNF-α及血管重构指标均呈正相关（P〈0. 01）,VGEF与TNF-α表达呈正相关（P〈0. 01）. 结论：VGEF及TNF-α均可能参与慢性血栓栓塞性肺动脉高压、肺血管重构的病理生理过程,炎症反应是慢性肺动脉高压形成重要机制之一.To study chronic thromboembolic pulmonary hypertension （ chronic thrombo embolic pulmonary hypertension, CTEPH） Changes in the expression levels of inflammatory cytokines in order to assess the relationship between pulmonary vascular remode-ling inflammatory response. Methods：64 male Wistar rats were randomly divided into 8 groups of 8： initial control group （ IC group） , embolism 3 d group, one week group, 2 weeks, 4 weeks, 8 weeks group, 12 weeks group （D3, W1, W2, W4, W8, W12）, the terminal group （ EC group）. Preparation of blood of about 0. 5 mm size autologous thrombus, jugular vein injection of 27 to 30 autologous thrombus, the second injection the same number thrombosis two weeks after two intramuscular gentamicin prevention of infection after sur-gery, throughout the intraperitoneal injection of tranexamic acid. Up to the target date after each set of experiments, the rats measured mean pulmonary arterial pressure （ mPAP） , pulmonary relative thickness （ PAMT） , wall area / total area （ WA / TA） percentage immu-nohistochemistry vascular endothelial growth factor （ VGEF） , tumor necrosis factor （ TNF-α） expression. Results：After the first embo-lization mPAP increased slightly, no significant difference （P〉 0. 05） with the control group, after the second embolization mPAP signifi-cantly increased with time （all P 〈0. 01）;PAMT , WA / TA in four weeks after the extension was significantly increased over time （4 weeks group P 〈0. 05,8 week, 12 weeks group P 〈0. 01）;serum at different times VGEF, TNF-αthan the initial value of the control group and terminal control group were significantly higher, P 〈0. 01, the difference was statistically significant, which reached its peak W1, then declined again after the Second embolization W4 peaked, then maintain a high level. Correlation analysis showed that, VGEF positively correlated with TNF-α and remodeling index showed （P 〈0. 01）, VGEF TNF-αexpression was positively correlated （P 〈0. O1）

关 键 词：血栓栓塞 肺动脉高压 炎症反应 肺血管重构 

分 类 号：R543.2[医药卫生—心血管疾病]